Giuseppe
Emeritus
[Source: Antimicrobial Agents and Chemotherapy, full text: (LINK). Abstract, edited.]
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Novel combinations of vancomycin plus ceftaroline or oxacillin against methicillin resistant vancomycin-intermediate Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA).
B.J. Werth 1, C. Vidaillac 1, K.P. Murray 1, K.L. Newton 1, G. Sakoulas 3,4, P. Nonejuie 5, J. Pogliano 5 and M.J. Rybak 1,2
Author Affiliations: <SUP>1</SUP>Anti-Infective Research Laboratory, Eugene Applebaum College of Pharmacy and Health Sciences <SUP>2</SUP>School of Medicine <SUP>3</SUP>Wayne State University, Detroit, MI 48201 <SUP>4</SUP>Department of Pediatric Pharmacology and Drug Discovery, University of California San Diego School of Medicine, La Jolla, CA USA <SUP>5</SUP>University of California San Diego Division of Biology, La Jolla, CA USA
ABSTRACT
We demonstrated a significant inverse correlation between vancomycin and beta-lactam susceptibility in vancomycin-intermediate Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA) isolates. Using time kill assays, vancomycin plus oxacillin or ceftaroline was synergistic against 3/5 VISA and 1/5 hVISA or 5/5 VISA and 4/5 hVISA, respectively. Beta-lactam exposure reduced overall vancomycin-bodipy binding but may have improved vancomycin-cell wall interactions to improve vancomycin activity. Further research is warranted to elucidate the mechanism behind vancomycin and beta-lactam synergy.
FOOTNOTES
Corresponding Author: Michael J. Rybak, Anti-Infective Research Laboratory, Pharmacy Practice ? 4148, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Ave., Detroit, MI 48201, Tel: (313) 577-4376, Fax: (313) 577-8915, Email: m.rybak@wayne.edu
Copyright ? 2013, American Society for Microbiology. All Rights Reserved.
-B.J. Werth 1, C. Vidaillac 1, K.P. Murray 1, K.L. Newton 1, G. Sakoulas 3,4, P. Nonejuie 5, J. Pogliano 5 and M.J. Rybak 1,2
Author Affiliations: <SUP>1</SUP>Anti-Infective Research Laboratory, Eugene Applebaum College of Pharmacy and Health Sciences <SUP>2</SUP>School of Medicine <SUP>3</SUP>Wayne State University, Detroit, MI 48201 <SUP>4</SUP>Department of Pediatric Pharmacology and Drug Discovery, University of California San Diego School of Medicine, La Jolla, CA USA <SUP>5</SUP>University of California San Diego Division of Biology, La Jolla, CA USA
ABSTRACT
We demonstrated a significant inverse correlation between vancomycin and beta-lactam susceptibility in vancomycin-intermediate Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA) isolates. Using time kill assays, vancomycin plus oxacillin or ceftaroline was synergistic against 3/5 VISA and 1/5 hVISA or 5/5 VISA and 4/5 hVISA, respectively. Beta-lactam exposure reduced overall vancomycin-bodipy binding but may have improved vancomycin-cell wall interactions to improve vancomycin activity. Further research is warranted to elucidate the mechanism behind vancomycin and beta-lactam synergy.
FOOTNOTES
Corresponding Author: Michael J. Rybak, Anti-Infective Research Laboratory, Pharmacy Practice ? 4148, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Ave., Detroit, MI 48201, Tel: (313) 577-4376, Fax: (313) 577-8915, Email: m.rybak@wayne.edu
Copyright ? 2013, American Society for Microbiology. All Rights Reserved.
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