tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2018 Sep 14. doi: 10.1111/irv.12610. [Epub ahead of print]
[h=1]Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016.[/h] Bolton MJ[SUP]1[/SUP], Abente EJ[SUP]1[/SUP], Venkatesh D[SUP]2[/SUP], Stratton JA[SUP]1[/SUP], Zeller M[SUP]1,[/SUP][SUP]3[/SUP], Anderson TK[SUP]1[/SUP], Lewis NS[SUP]2[/SUP], Vincent AL[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Six amino acid positions (145, 155, 156, 158, 159 and 189, referred to as the antigenic motif; H3 numbering) in the globular head region of hemagglutinin (HA1 domain) play an important role in defining the antigenic phenotype of swine Clade IV (C-IV) H3N2 IAV, containing an H3 from a late 1990s human-to-swine introduction. We hypothesized that antigenicity of a swine C-IV H3 virus could be inferred based upon the antigenic motif if it matched a previously characterized antigen with the same motif. An increasing number of C-IV H3 genes encoding antigenic motifs that had not been previously characterized were observed in the U.S. pig population between 2012-2016.
[h=4]OBJECTIVES:[/h] A broad panel of contemporary H3 viruses with uncharacterized antigenic motifs were selected across multiple clades within C-IV to assess the impact of HA1 genetic diversity on the antigenic phenotype.
[h=4]METHODS:[/h] Hemagglutination inhibition (HI) assays were performed with isolates selected based on antigenic motif, tested against a panel of swine anti-sera, and visualized by antigenic cartography.
[h=4]RESULTS:[/h] A previously uncharacterized motif with low but sustained circulation in the swine population demonstrated a distinct phenotype from those previously characterized. Antigenic variation increased for viruses with similar antigenic motifs, likely due to amino acid substitutions outside the motif.
[h=4]CONCLUSIONS:[/h] Although antigenic motifs were largely associated with antigenic distances, substantial diversity among co-circulating viruses poses a significant challenge for effective vaccine development. Continued surveillance and antigenic characterization of circulating strains is critical for improving vaccine efforts to control C-IV H3 IAV in U.S. swine. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] H3N2; antigenic cartography; antigenic evolution; influenza A virus; swine
PMID: 30216671 DOI: 10.1111/irv.12610
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[h=1]Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016.[/h] Bolton MJ[SUP]1[/SUP], Abente EJ[SUP]1[/SUP], Venkatesh D[SUP]2[/SUP], Stratton JA[SUP]1[/SUP], Zeller M[SUP]1,[/SUP][SUP]3[/SUP], Anderson TK[SUP]1[/SUP], Lewis NS[SUP]2[/SUP], Vincent AL[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Six amino acid positions (145, 155, 156, 158, 159 and 189, referred to as the antigenic motif; H3 numbering) in the globular head region of hemagglutinin (HA1 domain) play an important role in defining the antigenic phenotype of swine Clade IV (C-IV) H3N2 IAV, containing an H3 from a late 1990s human-to-swine introduction. We hypothesized that antigenicity of a swine C-IV H3 virus could be inferred based upon the antigenic motif if it matched a previously characterized antigen with the same motif. An increasing number of C-IV H3 genes encoding antigenic motifs that had not been previously characterized were observed in the U.S. pig population between 2012-2016.
[h=4]OBJECTIVES:[/h] A broad panel of contemporary H3 viruses with uncharacterized antigenic motifs were selected across multiple clades within C-IV to assess the impact of HA1 genetic diversity on the antigenic phenotype.
[h=4]METHODS:[/h] Hemagglutination inhibition (HI) assays were performed with isolates selected based on antigenic motif, tested against a panel of swine anti-sera, and visualized by antigenic cartography.
[h=4]RESULTS:[/h] A previously uncharacterized motif with low but sustained circulation in the swine population demonstrated a distinct phenotype from those previously characterized. Antigenic variation increased for viruses with similar antigenic motifs, likely due to amino acid substitutions outside the motif.
[h=4]CONCLUSIONS:[/h] Although antigenic motifs were largely associated with antigenic distances, substantial diversity among co-circulating viruses poses a significant challenge for effective vaccine development. Continued surveillance and antigenic characterization of circulating strains is critical for improving vaccine efforts to control C-IV H3 IAV in U.S. swine. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] H3N2; antigenic cartography; antigenic evolution; influenza A virus; swine
PMID: 30216671 DOI: 10.1111/irv.12610
Free full text