tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2013 Dec;7 Suppl 4:52-60. doi: 10.1111/irv.12190.
Antigenic drift of H1N1 influenza A virus in pigs with and without passive immunity.
Diaz A, Allerson M, Culhane M, Sreevatsan S, Torremorell M.
Author information
Abstract
BACKGROUND:
The genetic and antigenic characteristics of influenza A viruses (IAV) within and between species change over time due to antigenic shift and drift. Although pigs are known to play a key role in the epidemiology of IAV between species, little is known about the molecular evolution of IAV hemagglutinin (HA) in pigs.
OBJECTIVES:
The aim of this study was to evaluate the HA drift of an H1N1 IAV after infecting weaned pigs with or without maternally derived passive immunity.
METHODS:
Three- to four-week-old piglets born either to vaccinated or unvaccinated sows were contact-infected upon exposure with an IAV-infected pig. Nasal swabs were collected daily from each pig and tested for IAV by RRT-PCR. Full-length HA sequences were obtained directly from positive nasal swabs and compared between groups.
RESULTS:
Synonymous and non-synonymous mutations were detected in pigs with and without passive immunity. Most of the non-synonymous mutations occurred within the HA1 region of the HA. Changes within HA1 region were only identified in antigenic site B in pigs without passive immunity and in antigenic sites A, B, and D in pigs with passive immunity. However, there was no association between the immune status of the pig and the amino acid substitutions observed.
CONCLUSIONS:
Overall, we demonstrated that amino acid substitutions within antigenic sites can happen in weaned pigs with or without passive immunity shortly after infection.
? 2013 Blackwell Publishing Ltd.
KEYWORDS:
Antigenic drift, Swine influenza, passive immunity
PMID:
24224820
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24224820
Antigenic drift of H1N1 influenza A virus in pigs with and without passive immunity.
Diaz A, Allerson M, Culhane M, Sreevatsan S, Torremorell M.
Author information
Abstract
BACKGROUND:
The genetic and antigenic characteristics of influenza A viruses (IAV) within and between species change over time due to antigenic shift and drift. Although pigs are known to play a key role in the epidemiology of IAV between species, little is known about the molecular evolution of IAV hemagglutinin (HA) in pigs.
OBJECTIVES:
The aim of this study was to evaluate the HA drift of an H1N1 IAV after infecting weaned pigs with or without maternally derived passive immunity.
METHODS:
Three- to four-week-old piglets born either to vaccinated or unvaccinated sows were contact-infected upon exposure with an IAV-infected pig. Nasal swabs were collected daily from each pig and tested for IAV by RRT-PCR. Full-length HA sequences were obtained directly from positive nasal swabs and compared between groups.
RESULTS:
Synonymous and non-synonymous mutations were detected in pigs with and without passive immunity. Most of the non-synonymous mutations occurred within the HA1 region of the HA. Changes within HA1 region were only identified in antigenic site B in pigs without passive immunity and in antigenic sites A, B, and D in pigs with passive immunity. However, there was no association between the immune status of the pig and the amino acid substitutions observed.
CONCLUSIONS:
Overall, we demonstrated that amino acid substitutions within antigenic sites can happen in weaned pigs with or without passive immunity shortly after infection.
? 2013 Blackwell Publishing Ltd.
KEYWORDS:
Antigenic drift, Swine influenza, passive immunity
PMID:
24224820
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24224820