tetano
Editor, Senior Moderator
Physiol Rep. 2016 Dec;4(24). pii: e13071. doi: 10.14814/phy2.13071.
[h=1]Anticoagulation increases alveolar hemorrhage in mice infected with influenza A.[/h] Tatsumi K[SUP]1[/SUP], Antoniak S[SUP]1,[/SUP][SUP]2[/SUP], Subramaniam S[SUP]1,[/SUP][SUP]2[/SUP], Gondouin B[SUP]1[/SUP], Neidich SD[SUP]3[/SUP], Beck MA[SUP]3[/SUP], Mickelson J[SUP]1[/SUP], Monroe DM 3rd[SUP]1[/SUP], Bastarache JA[SUP]4[/SUP], Mackman N[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus infection is a common respiratory tract infection. Alveolar hemorrhage has been reported in patients with influenza pneumonia and in mice infected with influenza A. In this study, we investigated the effect of two anticoagulants on alveolar hemorrhage after influenza A virus (IAV) infection of wild-type mice. Wild-type mice were anticoagulated with either warfarin or the direct thrombin inhibitor dabigatran etexilate and then infected with a mouse-adapted influenza virus (A/Puerto Rico/8/34 H1N1). Alveolar hemorrhage was assessed by measuring hemoglobin levels in the bronchoalveolar lavage fluid (BALF). We also measured vascular permeability and viral genomes in the lung, as well as white blood cells, inflammatory mediators, and protein in BALF Survival and body weight were monitored for 14 days after influenza A infection. In infected mice receiving either warfarin or dabigatran etexilate we observed decreased activation of coagulation in the BALF and increased alveolar hemorrhage. Warfarin but not dabigatran etexilate increased vascular permeability and mortality of influenza A-infected mice. Anticoagulation did not affect levels of influenza A genomes, white blood cells, inflammatory mediators, or protein in the BALF Our study indicates that systemic anticoagulation increases alveolar hemorrhage in influenza A-infected mice.
? 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
[h=4]KEYWORDS:[/h] Alveolar hemorrhage; anticoagulation; influenza A virus infection; mouse model
PMID: 28003564 DOI: 10.14814/phy2.13071
[PubMed - in process]
[h=1]Anticoagulation increases alveolar hemorrhage in mice infected with influenza A.[/h] Tatsumi K[SUP]1[/SUP], Antoniak S[SUP]1,[/SUP][SUP]2[/SUP], Subramaniam S[SUP]1,[/SUP][SUP]2[/SUP], Gondouin B[SUP]1[/SUP], Neidich SD[SUP]3[/SUP], Beck MA[SUP]3[/SUP], Mickelson J[SUP]1[/SUP], Monroe DM 3rd[SUP]1[/SUP], Bastarache JA[SUP]4[/SUP], Mackman N[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus infection is a common respiratory tract infection. Alveolar hemorrhage has been reported in patients with influenza pneumonia and in mice infected with influenza A. In this study, we investigated the effect of two anticoagulants on alveolar hemorrhage after influenza A virus (IAV) infection of wild-type mice. Wild-type mice were anticoagulated with either warfarin or the direct thrombin inhibitor dabigatran etexilate and then infected with a mouse-adapted influenza virus (A/Puerto Rico/8/34 H1N1). Alveolar hemorrhage was assessed by measuring hemoglobin levels in the bronchoalveolar lavage fluid (BALF). We also measured vascular permeability and viral genomes in the lung, as well as white blood cells, inflammatory mediators, and protein in BALF Survival and body weight were monitored for 14 days after influenza A infection. In infected mice receiving either warfarin or dabigatran etexilate we observed decreased activation of coagulation in the BALF and increased alveolar hemorrhage. Warfarin but not dabigatran etexilate increased vascular permeability and mortality of influenza A-infected mice. Anticoagulation did not affect levels of influenza A genomes, white blood cells, inflammatory mediators, or protein in the BALF Our study indicates that systemic anticoagulation increases alveolar hemorrhage in influenza A-infected mice.
? 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
[h=4]KEYWORDS:[/h] Alveolar hemorrhage; anticoagulation; influenza A virus infection; mouse model
PMID: 28003564 DOI: 10.14814/phy2.13071
[PubMed - in process]