tetano
Editor, Senior Moderator
J Diabetes Investig. 2017 May 6. doi: 10.1111/jdi.12690. [Epub ahead of print]
[h=1]Antibodies against H1N1 influenza virus cross-react with alpha cells of pancreatic islets.[/h] Qi Z[SUP]1[/SUP], Hu H[SUP]1[/SUP], Wang Z[SUP]2[/SUP], Wang G[SUP]1[/SUP], Li Y[SUP]1[/SUP], Zhao X[SUP]1[/SUP], Feng Y[SUP]1[/SUP], Huo X[SUP]1[/SUP], Sun J[SUP]1[/SUP], Feng Q[SUP]1[/SUP], Liu Y[SUP]1[/SUP], Wang N[SUP]1[/SUP], Guo C[SUP]1[/SUP], Li Y[SUP]1[/SUP], Wang R[SUP]3[/SUP], Hu J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Epidemiological studies have documented that the incidence of human type 1 diabetes (T1D) is significantly increased after H1N1 epidemic. However, direct link between T1D and virus infection remains elusive. We generated 84 clones of murine mAbs against the H1N1 and performed immunohistochemistry in normal human tissue microarray. The results demonstrated that two clones specifically cross-reacted with human pancreatic tissues α cell of pancreatic islet. RT-PCR and DNA sequencing showed that the amino acid sequences of light and heavy chains of these clones were different. Importantly, the expression profiles of two mAbs were individual differences. We for the first time provide direct evidence that monoclonal antibodies against H1N1 can cross-react with human pancreas α cells, another source of β cells, suggesting α cells might be a novel target to be investigated in diabetes research. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Cross-reactivity; Monoclonal antibodies; Tissue microarrays
PMID: 28477380 DOI: 10.1111/jdi.12690
[h=1]Antibodies against H1N1 influenza virus cross-react with alpha cells of pancreatic islets.[/h] Qi Z[SUP]1[/SUP], Hu H[SUP]1[/SUP], Wang Z[SUP]2[/SUP], Wang G[SUP]1[/SUP], Li Y[SUP]1[/SUP], Zhao X[SUP]1[/SUP], Feng Y[SUP]1[/SUP], Huo X[SUP]1[/SUP], Sun J[SUP]1[/SUP], Feng Q[SUP]1[/SUP], Liu Y[SUP]1[/SUP], Wang N[SUP]1[/SUP], Guo C[SUP]1[/SUP], Li Y[SUP]1[/SUP], Wang R[SUP]3[/SUP], Hu J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Epidemiological studies have documented that the incidence of human type 1 diabetes (T1D) is significantly increased after H1N1 epidemic. However, direct link between T1D and virus infection remains elusive. We generated 84 clones of murine mAbs against the H1N1 and performed immunohistochemistry in normal human tissue microarray. The results demonstrated that two clones specifically cross-reacted with human pancreatic tissues α cell of pancreatic islet. RT-PCR and DNA sequencing showed that the amino acid sequences of light and heavy chains of these clones were different. Importantly, the expression profiles of two mAbs were individual differences. We for the first time provide direct evidence that monoclonal antibodies against H1N1 can cross-react with human pancreas α cells, another source of β cells, suggesting α cells might be a novel target to be investigated in diabetes research. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Cross-reactivity; Monoclonal antibodies; Tissue microarrays
PMID: 28477380 DOI: 10.1111/jdi.12690