tetano
Editor, Senior Moderator
Angew Chem Int Ed Engl
. 2021 Dec 22.
doi: 10.1002/anie.202112995. Online ahead of print.
A Charge-Switchable Zwitterionic Peptide for Rapid Detection of SARS-CoV-2 Main Protease
Zhicheng Jin[SUP] 1 [/SUP], Yash Mantri[SUP] 2 [/SUP], Maurice Retout[SUP] 3 [/SUP], Yong Cheng[SUP] 3 [/SUP], Jiajing Zhou[SUP] 3 [/SUP], Alec Jorns[SUP] 3 [/SUP], Pavla Fajtova[SUP] 4 [/SUP], Wonjun Yim[SUP] 5 [/SUP], Colman Moore[SUP] 3 [/SUP], Ming Xu[SUP] 3 [/SUP], Matthew Creyer[SUP] 3 [/SUP], Raina Borum[SUP] 3 [/SUP], Jingcheng Zhou[SUP] 3 [/SUP], Zhuohong Wu[SUP] 3 [/SUP], Tengyu He[SUP] 5 [/SUP], William Penny[SUP] 4 [/SUP], Anthony O'Donoghue[SUP] 4 [/SUP], Jesse Jokerst[SUP] 6 [/SUP]
Affiliations
Abstract
The transmission of SARS-CoV-2 coronavirus has led to the COVID-19 pandemic. Nucleic acid testing while specific has limitations for mass surveillance. One alternative is the main protease (Mpro) due to its functional importance in mediating the viral life cycle. Here, we describe a combination of modular substrate and gold colloids to detect Mpro via visual readout. The strategy involves zwitterionic peptide that carries opposite charges at the C-/N-terminus to exploit the specific recognition by Mpro. Autolytic cleavage releases positively-charged moiety that assembles the nanoparticles with rapid color changes (t <10 min). We determine a limit of detection for Mpro in breath condensate matrices <10 nM. We further assayed ten COVID-negative subjects and found no false-positive result. In the light of simplicity, our test for viral protease is not limited to an equipped laboratory, but also is amenable to integrating as portable point-of-care devices including those on face-coverings.
Keywords: COVID-19; colorimetric analysis; mpro/3clpro/nsp5; smart mask; zwitterionic peptide.
. 2021 Dec 22.
doi: 10.1002/anie.202112995. Online ahead of print.
A Charge-Switchable Zwitterionic Peptide for Rapid Detection of SARS-CoV-2 Main Protease
Zhicheng Jin[SUP] 1 [/SUP], Yash Mantri[SUP] 2 [/SUP], Maurice Retout[SUP] 3 [/SUP], Yong Cheng[SUP] 3 [/SUP], Jiajing Zhou[SUP] 3 [/SUP], Alec Jorns[SUP] 3 [/SUP], Pavla Fajtova[SUP] 4 [/SUP], Wonjun Yim[SUP] 5 [/SUP], Colman Moore[SUP] 3 [/SUP], Ming Xu[SUP] 3 [/SUP], Matthew Creyer[SUP] 3 [/SUP], Raina Borum[SUP] 3 [/SUP], Jingcheng Zhou[SUP] 3 [/SUP], Zhuohong Wu[SUP] 3 [/SUP], Tengyu He[SUP] 5 [/SUP], William Penny[SUP] 4 [/SUP], Anthony O'Donoghue[SUP] 4 [/SUP], Jesse Jokerst[SUP] 6 [/SUP]
Affiliations
- PMID: 34936725
- DOI: 10.1002/anie.202112995
Abstract
The transmission of SARS-CoV-2 coronavirus has led to the COVID-19 pandemic. Nucleic acid testing while specific has limitations for mass surveillance. One alternative is the main protease (Mpro) due to its functional importance in mediating the viral life cycle. Here, we describe a combination of modular substrate and gold colloids to detect Mpro via visual readout. The strategy involves zwitterionic peptide that carries opposite charges at the C-/N-terminus to exploit the specific recognition by Mpro. Autolytic cleavage releases positively-charged moiety that assembles the nanoparticles with rapid color changes (t <10 min). We determine a limit of detection for Mpro in breath condensate matrices <10 nM. We further assayed ten COVID-negative subjects and found no false-positive result. In the light of simplicity, our test for viral protease is not limited to an equipped laboratory, but also is amenable to integrating as portable point-of-care devices including those on face-coverings.
Keywords: COVID-19; colorimetric analysis; mpro/3clpro/nsp5; smart mask; zwitterionic peptide.