tetano
Editor, Senior Moderator
Anal Methods
. 2024 Apr 18.
doi: 10.1039/d3ay02075d. Online ahead of print. Characterizing a visual lateral flow device for rapid SARS-CoV-2 virus protein detection: pre-clinical and system assessment
Natpapas Wiriyachaiporn[SUP] 1 [/SUP], Jetnapang Kongrueng[SUP] 1 [/SUP], Kannika Sukkuea[SUP] 1 [/SUP], Rattana Tanrattanawong[SUP] 1 [/SUP], Jarunee Vanichtanankul[SUP] 2 [/SUP], Thanaya Saeyang[SUP] 2 [/SUP], Tararat Jantra[SUP] 2 [/SUP], Deanpen Japrung[SUP] 1 [/SUP], Weerakanya Maneeprakorn[SUP] 1 [/SUP], Suwussa Bamrungsap[SUP] 1 [/SUP], Pareena Janchompoo[SUP] 3 [/SUP], Ekawat Pasomsub[SUP] 3 [/SUP]
Affiliations
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections have affected more than 769 million individuals worldwide over the last few years. Although the pandemic is transitioning into an endemic, the COVID-19 outbreak is still a global concern. A rapid screening platform is needed for effective preventive and control measures. Herein, a visual rapid lateral flow platform for SARS-CoV-2 nucleocapsid protein detection is developed. Under optimal conditions, the system demonstrated good detection sensitivity and selectivity against tested respiratory viruses. The system provides direct visual detection with a limit of 0.7 ng of the nucleocapsid protein per mL of a sample (0.7 ng mL[SUP]-1[/SUP]) within 15 minutes. Further, a correlation between direct visual detection and semi-quantitative analysis using a reader showed a similar detection limit (R[SUP]2[/SUP] = 0.9571). The repeatability and reproducibility studies highlighted the potential of the system for the rapid screening of SARS-CoV-2 infection, with variations within 5% and 10% at high and low protein concentrations, respectively. Subsequent pre-clinical validation to correlate the performance with the standard molecular approach (RT-PCR) using 170 nasopharyngeal swabs demonstrated 98% estimated sensitivity (95% CI, 89.35-99.95%) and 100% specificity (95% CI, 96.38-100%). The positive and negative predictive values were reported to be 100% and 99%, respectively, with an accuracy of 99.3%. With high viral load samples (Ct value ≤25, n = 47), the system demonstrated 100% detection sensitivity and specificity. The proposed technique provides a valuable platform for potential use in rapid screening, particularly during pandemics, where diagnostic capacity and mass screening are crucial.
. 2024 Apr 18.
doi: 10.1039/d3ay02075d. Online ahead of print. Characterizing a visual lateral flow device for rapid SARS-CoV-2 virus protein detection: pre-clinical and system assessment
Natpapas Wiriyachaiporn[SUP] 1 [/SUP], Jetnapang Kongrueng[SUP] 1 [/SUP], Kannika Sukkuea[SUP] 1 [/SUP], Rattana Tanrattanawong[SUP] 1 [/SUP], Jarunee Vanichtanankul[SUP] 2 [/SUP], Thanaya Saeyang[SUP] 2 [/SUP], Tararat Jantra[SUP] 2 [/SUP], Deanpen Japrung[SUP] 1 [/SUP], Weerakanya Maneeprakorn[SUP] 1 [/SUP], Suwussa Bamrungsap[SUP] 1 [/SUP], Pareena Janchompoo[SUP] 3 [/SUP], Ekawat Pasomsub[SUP] 3 [/SUP]
Affiliations
- PMID: 38634326
- DOI: 10.1039/d3ay02075d
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections have affected more than 769 million individuals worldwide over the last few years. Although the pandemic is transitioning into an endemic, the COVID-19 outbreak is still a global concern. A rapid screening platform is needed for effective preventive and control measures. Herein, a visual rapid lateral flow platform for SARS-CoV-2 nucleocapsid protein detection is developed. Under optimal conditions, the system demonstrated good detection sensitivity and selectivity against tested respiratory viruses. The system provides direct visual detection with a limit of 0.7 ng of the nucleocapsid protein per mL of a sample (0.7 ng mL[SUP]-1[/SUP]) within 15 minutes. Further, a correlation between direct visual detection and semi-quantitative analysis using a reader showed a similar detection limit (R[SUP]2[/SUP] = 0.9571). The repeatability and reproducibility studies highlighted the potential of the system for the rapid screening of SARS-CoV-2 infection, with variations within 5% and 10% at high and low protein concentrations, respectively. Subsequent pre-clinical validation to correlate the performance with the standard molecular approach (RT-PCR) using 170 nasopharyngeal swabs demonstrated 98% estimated sensitivity (95% CI, 89.35-99.95%) and 100% specificity (95% CI, 96.38-100%). The positive and negative predictive values were reported to be 100% and 99%, respectively, with an accuracy of 99.3%. With high viral load samples (Ct value ≤25, n = 47), the system demonstrated 100% detection sensitivity and specificity. The proposed technique provides a valuable platform for potential use in rapid screening, particularly during pandemics, where diagnostic capacity and mass screening are crucial.