tetano
Editor, Senior Moderator
Anal Methods
. 2024 Jul 11.
doi: 10.1039/d4ay01016g. Online ahead of print. A label-free H1N1 influenza virus immunosensor based on an N-LIG/Au laser induced graphene microelectrode
Yuchen Zhou[SUP] 1 2 [/SUP], Wanchun Chen[SUP] 2 [/SUP], Guangyuan Wang[SUP] 2 [/SUP], Zhenfeng Lei[SUP] 2 [/SUP], Mei Zhang[SUP] 2 [/SUP], Yanxia Li[SUP] 2 [/SUP]
Affiliations
A label-free immunosensor based on an N-doped laser direct graphene (N-LIG)/Au electrode was proposed for H1N1 influenza virus detection. By utilizing the instantaneous high temperature of laser irradiation, N atoms are generated by the decomposition of melamine dripped onto the surface of an LIG electrode to obtain N-LIG with higher conductivity. The doping of N atoms provides a large number of active sites for LIG microelectrodes. Combined with the electrodeposition of Au NPs, and covalently crosslinking antibodies, a simple, highly sensitive and stable immunosensing interface is constructed. The proposed H1N1 influenza virus immunosensor has a detection range of 0.01 fg mL[SUP]-1[/SUP] to 10 ng mL[SUP]-1[/SUP] with a detection limit as low as 0.004 fg mL[SUP]-1[/SUP]. The constructed sensor has ultra-high sensitivity and good selectivity and can be used for complex biological sample analysis, with potential application prospects in preventing the large-scale spread of influenza. Taking advantage of N-LIG electrode's properties will provide opportunities for developing portable electrochemical biosensors for health and environmental applications.
. 2024 Jul 11.
doi: 10.1039/d4ay01016g. Online ahead of print. A label-free H1N1 influenza virus immunosensor based on an N-LIG/Au laser induced graphene microelectrode
Yuchen Zhou[SUP] 1 2 [/SUP], Wanchun Chen[SUP] 2 [/SUP], Guangyuan Wang[SUP] 2 [/SUP], Zhenfeng Lei[SUP] 2 [/SUP], Mei Zhang[SUP] 2 [/SUP], Yanxia Li[SUP] 2 [/SUP]
Affiliations
- PMID: 38989680
- DOI: 10.1039/d4ay01016g
A label-free immunosensor based on an N-doped laser direct graphene (N-LIG)/Au electrode was proposed for H1N1 influenza virus detection. By utilizing the instantaneous high temperature of laser irradiation, N atoms are generated by the decomposition of melamine dripped onto the surface of an LIG electrode to obtain N-LIG with higher conductivity. The doping of N atoms provides a large number of active sites for LIG microelectrodes. Combined with the electrodeposition of Au NPs, and covalently crosslinking antibodies, a simple, highly sensitive and stable immunosensing interface is constructed. The proposed H1N1 influenza virus immunosensor has a detection range of 0.01 fg mL[SUP]-1[/SUP] to 10 ng mL[SUP]-1[/SUP] with a detection limit as low as 0.004 fg mL[SUP]-1[/SUP]. The constructed sensor has ultra-high sensitivity and good selectivity and can be used for complex biological sample analysis, with potential application prospects in preventing the large-scale spread of influenza. Taking advantage of N-LIG electrode's properties will provide opportunities for developing portable electrochemical biosensors for health and environmental applications.