tetano
Editor, Senior Moderator
Anal Chim Acta
. 2022 May 22;1208:339846.
doi: 10.1016/j.aca.2022.339846. Epub 2022 Apr 20.
Target-triggered cascade signal amplification for sensitive electrochemical detection of SARS-CoV-2 with clinical application
Ying Deng[SUP] 1 [/SUP], Ying Peng[SUP] 1 [/SUP], Lei Wang[SUP] 1 [/SUP], Minghui Wang[SUP] 1 [/SUP], Tianci Zhou[SUP] 1 [/SUP], Liangliang Xiang[SUP] 2 [/SUP], Jinlong Li[SUP] 3 [/SUP], Jie Yang[SUP] 1 [/SUP], Genxi Li[SUP] 4 [/SUP]
Affiliations
Abstract
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the outbreak of the 2019 coronavirus (COVID-19) disease, which greatly challenges the global economy and health. Simple and sensitive diagnosis of COVID-19 at the early stage is important to prevent the spread of pandemics. Herein, we have proposed a target-triggered cascade signal amplification in this work for sensitive analysis of SARS-CoV-2 RNA. Specifically, the presence of SARS-CoV-2 RNA can trigger the catalytic hairpin assembly to generate plenty of DNA duplexes with free 3'-OH termini, which can be recognized and catalyzed by the terminal deoxynucleotidyl transferase (TdT) to generate long strand DNA. The prolonged DNA can absorb substantial Ru(NH[SUB]3[/SUB])[SUB]6[/SUB][SUP]3+[/SUP] molecules via electrostatic interaction and produce an enhanced current response. The incorporation of catalytic hairpin assembly and TdT-mediated polymerization effectively lowers the detection limit to 45 fM, with a wide linear range from 0.1 pM to 3000 pM. Moreover, the proposed strategy possesses excellent selectivity to distinguish target RNA with single-base mismatched, three-base mismatched, and random sequences. Notably, the proposed electrochemical biosensor can be applied to analyze targets in complex circumstances containing 10% saliva, which implies its high stability and anti-interference. Moreover, the proposed strategy has been successfully applied to SARS CoV-2 RNA detection in clinical samples and may have the potential to be cultivated as an effective tool for COVID-19 diagnosis.
Keywords: COVID-19; Cascade signal amplification; Electrochemical biosensor; SARS-CoV-2 RNA.
. 2022 May 22;1208:339846.
doi: 10.1016/j.aca.2022.339846. Epub 2022 Apr 20.
Target-triggered cascade signal amplification for sensitive electrochemical detection of SARS-CoV-2 with clinical application
Ying Deng[SUP] 1 [/SUP], Ying Peng[SUP] 1 [/SUP], Lei Wang[SUP] 1 [/SUP], Minghui Wang[SUP] 1 [/SUP], Tianci Zhou[SUP] 1 [/SUP], Liangliang Xiang[SUP] 2 [/SUP], Jinlong Li[SUP] 3 [/SUP], Jie Yang[SUP] 1 [/SUP], Genxi Li[SUP] 4 [/SUP]
Affiliations
- PMID: 35525596
- DOI: 10.1016/j.aca.2022.339846
Abstract
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the outbreak of the 2019 coronavirus (COVID-19) disease, which greatly challenges the global economy and health. Simple and sensitive diagnosis of COVID-19 at the early stage is important to prevent the spread of pandemics. Herein, we have proposed a target-triggered cascade signal amplification in this work for sensitive analysis of SARS-CoV-2 RNA. Specifically, the presence of SARS-CoV-2 RNA can trigger the catalytic hairpin assembly to generate plenty of DNA duplexes with free 3'-OH termini, which can be recognized and catalyzed by the terminal deoxynucleotidyl transferase (TdT) to generate long strand DNA. The prolonged DNA can absorb substantial Ru(NH[SUB]3[/SUB])[SUB]6[/SUB][SUP]3+[/SUP] molecules via electrostatic interaction and produce an enhanced current response. The incorporation of catalytic hairpin assembly and TdT-mediated polymerization effectively lowers the detection limit to 45 fM, with a wide linear range from 0.1 pM to 3000 pM. Moreover, the proposed strategy possesses excellent selectivity to distinguish target RNA with single-base mismatched, three-base mismatched, and random sequences. Notably, the proposed electrochemical biosensor can be applied to analyze targets in complex circumstances containing 10% saliva, which implies its high stability and anti-interference. Moreover, the proposed strategy has been successfully applied to SARS CoV-2 RNA detection in clinical samples and may have the potential to be cultivated as an effective tool for COVID-19 diagnosis.
Keywords: COVID-19; Cascade signal amplification; Electrochemical biosensor; SARS-CoV-2 RNA.