tetano
Editor, Senior Moderator
Anal Chem
. 2022 Oct 17.
doi: 10.1021/acs.analchem.2c03606. Online ahead of print.
Smartphone-Based Electrochemical Immunoassay for Point-of-Care Detection of SARS-CoV-2 Nucleocapsid Protein
Ruijin Zeng[SUP] 1 [/SUP], Minghao Qiu[SUP] 1 [/SUP], Qing Wan[SUP] 2 [/SUP], Zhisheng Huang[SUP] 2 [/SUP], Xiaolong Liu[SUP] 3 [/SUP], Dianping Tang[SUP] 1 [/SUP], Dietmar Knopp[SUP] 4 [/SUP]
Affiliations
Abstract
Large-scale, rapid, and inexpensive serological diagnoses of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) are of great interest in reducing virus transmission at the population level; however, their development is greatly plagued by the lack of available point-of-care methods, leading to low detection efficiency. Herein, an ultrasensitive smartphone-based electrochemical immunoassay is reported for rapid (less than 5 min), low-cost, easy-to-implement detection of the SARS-CoV-2 nucleocapsid protein (SARS-CoV-2 N protein). Specifically, the electrochemical immunoassay was fabricated on a screen-printed carbon electrode coated with electrodeposited gold nanoparticles, followed by incubation of anti-N antibody (Ab) and bovine serum albumin as the working electrode. Accompanied by the antigen-antibody reaction between the SARS-CoV-2 N protein and the Ab, the electron transfer between the electroactive species [Fe(CN)[SUB]6[/SUB]][SUP]3-[/SUP]/[SUP]4-[/SUP] and the electrode surface is disturbed, resulting in reduced square-wave voltammetry currents at 0.075 V versus the Ag/AgCl reference electrode. The proposed immunoassay provided a good linear range with SARS-CoV-2 N protein concentrations within the scope of 0.01-1000 ng/mL (R[SUP]2[/SUP] = 0.9992) and the limit of detection down to 2.6 pg/mL. Moreover, the detection data are wirelessly transmitted to the interface of the smartphone, and the corresponding SARS-CoV-2 N protein concentration value is calculated and displayed. Therefore, the proposed portable detection mode offers great potential for self-differential diagnosis of residents, which will greatly facilitate the effective control and large-scale screening of virus transmission in resource-limited areas.
. 2022 Oct 17.
doi: 10.1021/acs.analchem.2c03606. Online ahead of print.
Smartphone-Based Electrochemical Immunoassay for Point-of-Care Detection of SARS-CoV-2 Nucleocapsid Protein
Ruijin Zeng[SUP] 1 [/SUP], Minghao Qiu[SUP] 1 [/SUP], Qing Wan[SUP] 2 [/SUP], Zhisheng Huang[SUP] 2 [/SUP], Xiaolong Liu[SUP] 3 [/SUP], Dianping Tang[SUP] 1 [/SUP], Dietmar Knopp[SUP] 4 [/SUP]
Affiliations
- PMID: 36251341
- DOI: 10.1021/acs.analchem.2c03606
Abstract
Large-scale, rapid, and inexpensive serological diagnoses of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) are of great interest in reducing virus transmission at the population level; however, their development is greatly plagued by the lack of available point-of-care methods, leading to low detection efficiency. Herein, an ultrasensitive smartphone-based electrochemical immunoassay is reported for rapid (less than 5 min), low-cost, easy-to-implement detection of the SARS-CoV-2 nucleocapsid protein (SARS-CoV-2 N protein). Specifically, the electrochemical immunoassay was fabricated on a screen-printed carbon electrode coated with electrodeposited gold nanoparticles, followed by incubation of anti-N antibody (Ab) and bovine serum albumin as the working electrode. Accompanied by the antigen-antibody reaction between the SARS-CoV-2 N protein and the Ab, the electron transfer between the electroactive species [Fe(CN)[SUB]6[/SUB]][SUP]3-[/SUP]/[SUP]4-[/SUP] and the electrode surface is disturbed, resulting in reduced square-wave voltammetry currents at 0.075 V versus the Ag/AgCl reference electrode. The proposed immunoassay provided a good linear range with SARS-CoV-2 N protein concentrations within the scope of 0.01-1000 ng/mL (R[SUP]2[/SUP] = 0.9992) and the limit of detection down to 2.6 pg/mL. Moreover, the detection data are wirelessly transmitted to the interface of the smartphone, and the corresponding SARS-CoV-2 N protein concentration value is calculated and displayed. Therefore, the proposed portable detection mode offers great potential for self-differential diagnosis of residents, which will greatly facilitate the effective control and large-scale screening of virus transmission in resource-limited areas.