tetano
Editor, Senior Moderator
Am J Trop Med Hyg
. 2022 Feb 7;tpmd210980.
doi: 10.4269/ajtmh.21-0980. Online ahead of print.
Nasopharyngeal Viral and Bacterial Co-Detection among Children from Low- and Middle-Income Countries with and without Pneumonia
Cédric Dananché, Gláucia Paranhos-Baccalà, Mélina Messaoudi, Mariam Sylla, Shally Awasthi, Ashish Bavdekar, Jean-William Pape, Vanessa Rouzier, Jianwei Wang, Sonali Sanghavi, Souleymane Diallo, Monidarin Chou, Tekchheng Eap, Mala Rakoto-Andrianarivelo, Hubert Endtz, Lili Ren, Budragchaagiin Dash-Yandag, Rosa Guillen, Pagbajabyn Nymadawa, Graciela Russomando, Florence Komurian-Pradel, Philippe Vanhems, Valentina Sánchez Picot, Pneumonia Global Approach for Biological Research, Infectious Diseases and Epidemics in Low-Income Countries (GABRIEL) Network
Abstract
The role of microbial coinfection in the pathogenesis of pneumonia in children is not well known. The aim of this work was to describe the prevalence of microorganism co-detection in nasopharyngeal samples (NPS) of pneumonia cases and control subjects and to study the potential association between nasopharyngeal microorganism co-detection and pneumonia. A case-control study was carried out from 2010 to 2014 in nine study sites located in low- or middle-income countries. The data from 888 children under 5 years of age with pneumonia (cases) and 870 children under 5 without pneumonia (controls) were analyzed. Nasopharyngeal samples were collected; reverse transcription polymerase chain reaction (RT-PCR) enabled the detection of five bacteria and 19 viruses. Multiple, mixed-effects logistic regression modeling was undertaken to evaluate the association between microorganism co-detection and pneumonia. A single Streptococcus pneumoniae colonization was observed in 15.2% of the controls and 10.1% of the cases (P = 0.001), whereas S. pneumoniae and a single virus co-detection was observed in 33.3% of the cases and in 14.6% of the controls (P < 0.001). Co-detections with rhinovirus, respiratory syncytial virus, parainfluenza virus, human metapneumovirus, and influenza virus were more frequent in the cases compared with the controls (P < 0.001) and were significantly associated with pneumonia in multiple regression analysis. The proportion of single virus detection without bacterial co-detection was not different between cases and controls (13.6% versus 11.3%, P = 0.13). This study suggests that coinfection of S. pneumoniae and certain viruses may play a role in the pathophysiology of pneumonia in children.
. 2022 Feb 7;tpmd210980.
doi: 10.4269/ajtmh.21-0980. Online ahead of print.
Nasopharyngeal Viral and Bacterial Co-Detection among Children from Low- and Middle-Income Countries with and without Pneumonia
Cédric Dananché, Gláucia Paranhos-Baccalà, Mélina Messaoudi, Mariam Sylla, Shally Awasthi, Ashish Bavdekar, Jean-William Pape, Vanessa Rouzier, Jianwei Wang, Sonali Sanghavi, Souleymane Diallo, Monidarin Chou, Tekchheng Eap, Mala Rakoto-Andrianarivelo, Hubert Endtz, Lili Ren, Budragchaagiin Dash-Yandag, Rosa Guillen, Pagbajabyn Nymadawa, Graciela Russomando, Florence Komurian-Pradel, Philippe Vanhems, Valentina Sánchez Picot, Pneumonia Global Approach for Biological Research, Infectious Diseases and Epidemics in Low-Income Countries (GABRIEL) Network
- PMID: 35130481
- DOI: 10.4269/ajtmh.21-0980
Abstract
The role of microbial coinfection in the pathogenesis of pneumonia in children is not well known. The aim of this work was to describe the prevalence of microorganism co-detection in nasopharyngeal samples (NPS) of pneumonia cases and control subjects and to study the potential association between nasopharyngeal microorganism co-detection and pneumonia. A case-control study was carried out from 2010 to 2014 in nine study sites located in low- or middle-income countries. The data from 888 children under 5 years of age with pneumonia (cases) and 870 children under 5 without pneumonia (controls) were analyzed. Nasopharyngeal samples were collected; reverse transcription polymerase chain reaction (RT-PCR) enabled the detection of five bacteria and 19 viruses. Multiple, mixed-effects logistic regression modeling was undertaken to evaluate the association between microorganism co-detection and pneumonia. A single Streptococcus pneumoniae colonization was observed in 15.2% of the controls and 10.1% of the cases (P = 0.001), whereas S. pneumoniae and a single virus co-detection was observed in 33.3% of the cases and in 14.6% of the controls (P < 0.001). Co-detections with rhinovirus, respiratory syncytial virus, parainfluenza virus, human metapneumovirus, and influenza virus were more frequent in the cases compared with the controls (P < 0.001) and were significantly associated with pneumonia in multiple regression analysis. The proportion of single virus detection without bacterial co-detection was not different between cases and controls (13.6% versus 11.3%, P = 0.13). This study suggests that coinfection of S. pneumoniae and certain viruses may play a role in the pathophysiology of pneumonia in children.