tetano
Editor, Senior Moderator
Am J Transplant
. 2020 Aug 10.
doi: 10.1111/ajt.16251. Online ahead of print.
In-depth virological assessment of kidney transplant recipients with COVID-19
Ilies Benotmane[SUP] 1 2 3 [/SUP], Gabriela Gautier Vargas[SUP] 1 [/SUP], Marie-Jos?e Wendling[SUP] 2 [/SUP], Peggy Perrin[SUP] 1 3 [/SUP], Aur?lie Velay[SUP] 2 3 [/SUP], Xavier Bassand[SUP] 1 [/SUP], Dimitri Bedo[SUP] 1 [/SUP], Clement Baldacini[SUP] 1 [/SUP], Mylene Sagnard[SUP] 1 [/SUP], Dogan Bozman[SUP] 1 [/SUP], Margaux Della Chiesa[SUP] 1 [/SUP], Morgane Solis[SUP] 2 3 [/SUP], Floriane Gallais[SUP] 2 3 [/SUP], No?lle Cognard[SUP] 1 [/SUP], Jerome Olagne[SUP] 1 [/SUP], H?lo?se Delagreverie[SUP] 2 [/SUP], Louise Gontard[SUP] 2 [/SUP], Baptiste Panaget[SUP] 2 [/SUP], David Marx[SUP] 1 [/SUP], Francoise Heibel[SUP] 1 [/SUP], Laura Braun[SUP] 1 [/SUP], Bruno Moulin[SUP] 1 3 [/SUP], Sophie Caillard[SUP] 1 3 [/SUP], Samira Fafi-Kremer[SUP] 2 3 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread widely, causing coronavirus disease 2019 (COVID-19) and significant mortality. However, data on viral loads and antibody kinetics in immunocompromised populations are lacking. We aimed to determine nasopharyngeal and plasma viral loads via RT-PCR and SARS-CoV-2 serology via ELISA and study their association with severe forms of COVID-19 and death in kidney transplant recipients. In this study we examined hospitalized kidney transplant recipients with non-severe (n = 21) and severe (n =19) COVID-19. SARS-CoV-2 nasopharyngeal and plasma viral load and serological response were evaluated based on outcomes and disease severity. Ten recipients (25%) displayed persistent viral shedding 30 days after symptom onset. The SARS-CoV-2 viral load of the upper respiratory tract was not associated with severe COVID-19, whereas the plasma viral load was associated with COVID-19 severity (p=0.010) and mortality (p=0.010). All patients harbored antibodies the second week after symptom onset that persisted for two months. We conclude that plasma viral load is associated with COVID-19 morbidity and mortality, whereas nasopharyngeal viral load is not. SARS-CoV-2 shedding is prolonged in kidney transplant recipients and the humoral response to SARS-CoV-2 does not show significant impairment in this series of transplant recipients.
. 2020 Aug 10.
doi: 10.1111/ajt.16251. Online ahead of print.
In-depth virological assessment of kidney transplant recipients with COVID-19
Ilies Benotmane[SUP] 1 2 3 [/SUP], Gabriela Gautier Vargas[SUP] 1 [/SUP], Marie-Jos?e Wendling[SUP] 2 [/SUP], Peggy Perrin[SUP] 1 3 [/SUP], Aur?lie Velay[SUP] 2 3 [/SUP], Xavier Bassand[SUP] 1 [/SUP], Dimitri Bedo[SUP] 1 [/SUP], Clement Baldacini[SUP] 1 [/SUP], Mylene Sagnard[SUP] 1 [/SUP], Dogan Bozman[SUP] 1 [/SUP], Margaux Della Chiesa[SUP] 1 [/SUP], Morgane Solis[SUP] 2 3 [/SUP], Floriane Gallais[SUP] 2 3 [/SUP], No?lle Cognard[SUP] 1 [/SUP], Jerome Olagne[SUP] 1 [/SUP], H?lo?se Delagreverie[SUP] 2 [/SUP], Louise Gontard[SUP] 2 [/SUP], Baptiste Panaget[SUP] 2 [/SUP], David Marx[SUP] 1 [/SUP], Francoise Heibel[SUP] 1 [/SUP], Laura Braun[SUP] 1 [/SUP], Bruno Moulin[SUP] 1 3 [/SUP], Sophie Caillard[SUP] 1 3 [/SUP], Samira Fafi-Kremer[SUP] 2 3 [/SUP]
Affiliations
- PMID: 32777130
- DOI: 10.1111/ajt.16251
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread widely, causing coronavirus disease 2019 (COVID-19) and significant mortality. However, data on viral loads and antibody kinetics in immunocompromised populations are lacking. We aimed to determine nasopharyngeal and plasma viral loads via RT-PCR and SARS-CoV-2 serology via ELISA and study their association with severe forms of COVID-19 and death in kidney transplant recipients. In this study we examined hospitalized kidney transplant recipients with non-severe (n = 21) and severe (n =19) COVID-19. SARS-CoV-2 nasopharyngeal and plasma viral load and serological response were evaluated based on outcomes and disease severity. Ten recipients (25%) displayed persistent viral shedding 30 days after symptom onset. The SARS-CoV-2 viral load of the upper respiratory tract was not associated with severe COVID-19, whereas the plasma viral load was associated with COVID-19 severity (p=0.010) and mortality (p=0.010). All patients harbored antibodies the second week after symptom onset that persisted for two months. We conclude that plasma viral load is associated with COVID-19 morbidity and mortality, whereas nasopharyngeal viral load is not. SARS-CoV-2 shedding is prolonged in kidney transplant recipients and the humoral response to SARS-CoV-2 does not show significant impairment in this series of transplant recipients.