tetano
Editor, Senior Moderator
Aging (Albany NY)
. 2022 Sep 21;14(undefined).
doi: 10.18632/aging.204299. Online ahead of print.
Humoral immunoresponse elicited against an adenoviral-based SARS-CoV-2 coronavirus vaccine in elderly patients
Rodrigo Hernán Tomas-Grau[SUP] 1 [/SUP], Carolina Maldonado-Galdeano[SUP] 2 [/SUP], Mónica Aguilar López[SUP] 3 [/SUP], Esteban Vera Pingitore[SUP] 1 [/SUP], Patricia Aznar[SUP] 4 [/SUP], María Elena Alcorta[SUP] 4 [/SUP], Eva María Del Mar Vélez[SUP] 4 [/SUP], Agustín Stagnetto[SUP] 1 [/SUP], Silvana Estefanía Soliz-Santander[SUP] 1 [/SUP], César Luís Ávila[SUP] 1 [/SUP], Sergio Benjamín Socias[SUP] 1 [/SUP], Dardo Costas[SUP] 4 [/SUP], Rossana Elena Chahla[SUP] 3 [/SUP], Gabriela Perdigón[SUP] 2 [/SUP], Rosana Nieves Chehín[SUP] 1 [/SUP], Diego Ploper[SUP] 1 [/SUP], Silvia Inés Cazorla[SUP] 2 [/SUP]
Affiliations
Abstract
The early sequencing of the SARS-CoV-2 viral genome allowed for a speedy development of effective vaccines against the virus. Nevertheless, age-related immunosenescence, the inability to mount strong immune responses, still represents a major obstacle. Here, in a group of 149 elderly volunteers (70-96 years old), evolution of the humoral immune response over time to Gam-COVID-Vac (Sputnik V), a vaccine based on heterologous recombinant adenovirus-26 (Ad26) and adenovirus-5 (Ad5) carrying the Spike genome, was analyzed by an anti-RBD ELISA. At 28 days post vaccination (dpv), a seroconversion rate of 91% was achieved, showing the importance of administering at least two doses of Gam-COVID-Vac to elicit a robust immune response, especially in elderly individuals without previous SARS-CoV-2 infection. Interestingly, IgG specific antibodies that reached their highest titers around 28 dpv (median = 740), persisted without significant decrease after 60 dpv (median = 650). After 90 dpv, IgG titers began to drop, and at 180 dpv only 44.7% of the elderly individuals remained with detectable anti-RBD IgG antibodies. No significant differences were observed in specific humoral immune responses between genders at early times point. However, at 60 dpv anti-RBD titers were more persistent in elderly females, and only dropped at 90 dpv (p < 0.0001). As expected, the highest antibodies titers were elicited in the youngest subgroup (70-74 years). Our results show that Gam-COVID-Vac was able to deal with the ageing of the immune system, eliciting a robust immune response in an elderly cohort, which lasted approximately 90 dpv at high levels, and protected against COVID-19.
Keywords: COVID-19; Gam-COVID-Vac; SARS-CoV-2; elderly; humoral immune response.
. 2022 Sep 21;14(undefined).
doi: 10.18632/aging.204299. Online ahead of print.
Humoral immunoresponse elicited against an adenoviral-based SARS-CoV-2 coronavirus vaccine in elderly patients
Rodrigo Hernán Tomas-Grau[SUP] 1 [/SUP], Carolina Maldonado-Galdeano[SUP] 2 [/SUP], Mónica Aguilar López[SUP] 3 [/SUP], Esteban Vera Pingitore[SUP] 1 [/SUP], Patricia Aznar[SUP] 4 [/SUP], María Elena Alcorta[SUP] 4 [/SUP], Eva María Del Mar Vélez[SUP] 4 [/SUP], Agustín Stagnetto[SUP] 1 [/SUP], Silvana Estefanía Soliz-Santander[SUP] 1 [/SUP], César Luís Ávila[SUP] 1 [/SUP], Sergio Benjamín Socias[SUP] 1 [/SUP], Dardo Costas[SUP] 4 [/SUP], Rossana Elena Chahla[SUP] 3 [/SUP], Gabriela Perdigón[SUP] 2 [/SUP], Rosana Nieves Chehín[SUP] 1 [/SUP], Diego Ploper[SUP] 1 [/SUP], Silvia Inés Cazorla[SUP] 2 [/SUP]
Affiliations
- PMID: 36152043
- DOI: 10.18632/aging.204299
Abstract
The early sequencing of the SARS-CoV-2 viral genome allowed for a speedy development of effective vaccines against the virus. Nevertheless, age-related immunosenescence, the inability to mount strong immune responses, still represents a major obstacle. Here, in a group of 149 elderly volunteers (70-96 years old), evolution of the humoral immune response over time to Gam-COVID-Vac (Sputnik V), a vaccine based on heterologous recombinant adenovirus-26 (Ad26) and adenovirus-5 (Ad5) carrying the Spike genome, was analyzed by an anti-RBD ELISA. At 28 days post vaccination (dpv), a seroconversion rate of 91% was achieved, showing the importance of administering at least two doses of Gam-COVID-Vac to elicit a robust immune response, especially in elderly individuals without previous SARS-CoV-2 infection. Interestingly, IgG specific antibodies that reached their highest titers around 28 dpv (median = 740), persisted without significant decrease after 60 dpv (median = 650). After 90 dpv, IgG titers began to drop, and at 180 dpv only 44.7% of the elderly individuals remained with detectable anti-RBD IgG antibodies. No significant differences were observed in specific humoral immune responses between genders at early times point. However, at 60 dpv anti-RBD titers were more persistent in elderly females, and only dropped at 90 dpv (p < 0.0001). As expected, the highest antibodies titers were elicited in the youngest subgroup (70-74 years). Our results show that Gam-COVID-Vac was able to deal with the ageing of the immune system, eliciting a robust immune response in an elderly cohort, which lasted approximately 90 dpv at high levels, and protected against COVID-19.
Keywords: COVID-19; Gam-COVID-Vac; SARS-CoV-2; elderly; humoral immune response.