tetano
Editor, Senior Moderator
ADMET DMPK
. 2023 Dec 6;12(1):193-208.
doi: 10.5599/admet.2079. eCollection 2024. Antibody-labelled gold nanoparticles synthesized by laser ablation to detect SARS-CoV-2 antigen spike
Asri Sulfianti[SUP] 1 [/SUP], Vidhia Tiara Sopandi[SUP] 2 [/SUP], Isnaeni Isnaeni[SUP] 3 [/SUP], Jodi Suryanggono[SUP] 1 [/SUP], Sabar Pambudi[SUP] 1 [/SUP], Sjaikhurrizal El Muttaqien[SUP] 1 [/SUP], Febby Nurdiya Ningsih[SUP] 1 [/SUP], Tika Widayanti[SUP] 1 [/SUP], Etik Mardliyati[SUP] 1 [/SUP], Annisa Annisa[SUP] 2 [/SUP]
Affiliations
Background and purpose: Rapid detection test via lateral flow immunoassay (LFIA) is employed as an alternate method to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection. Gold nanoparticles (AuNPs), a vital component of LFIA, can be synthesized by laser ablation technique. This intense laser radiation may result in monodisperse gold nanoclusters, which are impurity-free and demonstrate innovative biocompatible surface chemistry. In this current research, laser-ablated AuNPs are produced and coupled with an anti-spike SARS-CoV-2 monoclonal antibody (mAb) generated in our prior study.
Experimental approach: The AuNPs from 30,000 shots of laser ablation exhibited a robust red color with a maximum absorbance peak at 520 nm. The performance of AuNPs-mAb conjugates as a signal reporter was then evaluated in half-stick LFIA.
Key results: The size distribution of AuNPs shows a relatively monodisperse and unimodal distribution with average particle diameters of 44.77 nm and a surface potential of -38.5 mV. The purified anti-spike mAb SARS-CoV-2 yielded two protein bands, representing the mAb heavy chain at 55 kDa and its light chain at 25 kDa. The immobilization of anti-spike mAb onto the surface of AuNPs revealed that 25 g/ml of mAb at phosphate buffer pH 9 was required to stabilize the AuNPs. The functional test of this conjugate was performed using dipstick LFIA, and the result shows that the AuNPs-mAb conjugates could successfully detect commercial spike antigen of SARS-CoV-2 at 10 ng level.
Conclusion: In this study, laser-ablated AuNPs were functionalized with anti-spike mAb SARS-CoV-2 and successfully used as a signal reporter in half-stick LFIA for detecting antigen spike SARS-CoV-2.
Keywords: Covid-19; anti-SARS-CoV-2; lateral flow immunoassay; monoclonal antibody.
. 2023 Dec 6;12(1):193-208.
doi: 10.5599/admet.2079. eCollection 2024. Antibody-labelled gold nanoparticles synthesized by laser ablation to detect SARS-CoV-2 antigen spike
Asri Sulfianti[SUP] 1 [/SUP], Vidhia Tiara Sopandi[SUP] 2 [/SUP], Isnaeni Isnaeni[SUP] 3 [/SUP], Jodi Suryanggono[SUP] 1 [/SUP], Sabar Pambudi[SUP] 1 [/SUP], Sjaikhurrizal El Muttaqien[SUP] 1 [/SUP], Febby Nurdiya Ningsih[SUP] 1 [/SUP], Tika Widayanti[SUP] 1 [/SUP], Etik Mardliyati[SUP] 1 [/SUP], Annisa Annisa[SUP] 2 [/SUP]
Affiliations
- PMID: 38560711
- PMCID: PMC10974819
- DOI: 10.5599/admet.2079
Background and purpose: Rapid detection test via lateral flow immunoassay (LFIA) is employed as an alternate method to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection. Gold nanoparticles (AuNPs), a vital component of LFIA, can be synthesized by laser ablation technique. This intense laser radiation may result in monodisperse gold nanoclusters, which are impurity-free and demonstrate innovative biocompatible surface chemistry. In this current research, laser-ablated AuNPs are produced and coupled with an anti-spike SARS-CoV-2 monoclonal antibody (mAb) generated in our prior study.
Experimental approach: The AuNPs from 30,000 shots of laser ablation exhibited a robust red color with a maximum absorbance peak at 520 nm. The performance of AuNPs-mAb conjugates as a signal reporter was then evaluated in half-stick LFIA.
Key results: The size distribution of AuNPs shows a relatively monodisperse and unimodal distribution with average particle diameters of 44.77 nm and a surface potential of -38.5 mV. The purified anti-spike mAb SARS-CoV-2 yielded two protein bands, representing the mAb heavy chain at 55 kDa and its light chain at 25 kDa. The immobilization of anti-spike mAb onto the surface of AuNPs revealed that 25 g/ml of mAb at phosphate buffer pH 9 was required to stabilize the AuNPs. The functional test of this conjugate was performed using dipstick LFIA, and the result shows that the AuNPs-mAb conjugates could successfully detect commercial spike antigen of SARS-CoV-2 at 10 ng level.
Conclusion: In this study, laser-ablated AuNPs were functionalized with anti-spike mAb SARS-CoV-2 and successfully used as a signal reporter in half-stick LFIA for detecting antigen spike SARS-CoV-2.
Keywords: Covid-19; anti-SARS-CoV-2; lateral flow immunoassay; monoclonal antibody.