tetano
Editor, Senior Moderator
ACS Sens
. 2023 Jan 27.
doi: 10.1021/acssensors.2c02512. Online ahead of print.
Multifunctional Nanoparticle Platform for Surface Accumulative Nucleic Acid Amplification and Rapid Electrochemical Detection: An Application to Pathogenic Coronavirus
Jeong Ook Soh[SUP] 1 2 [/SUP], Bum Chul Park[SUP] 3 4 [/SUP], Hyeon Su Park[SUP] 3 [/SUP], Myeong Soo Kim[SUP] 3 [/SUP], Hong En Fu[SUP] 3 [/SUP], Young Keun Kim[SUP] 3 4 [/SUP], Ju Hun Lee[SUP] 1 2 [/SUP]
Affiliations
Abstract
Of various molecular diagnostic assays, the real-time reverse transcription polymerase chain reaction is considered the gold standard for infection diagnosis, despite critical drawbacks that limit rapid detection and accessibility. To confront these issues, several nanoparticle-based molecular detection methods have been developed to a great extent, but still possess several challenges. In this study, a novel nucleic acid amplification method termed nanoparticle-based surface localized amplification (nSLAM) is paired with electrochemical detection (ECD) to develop a nucleic acid biosensor platform that overcomes these limitations. The system uses primer-functionalized Fe[SUB]3[/SUB]O[SUB]4[/SUB]-Au core-shell nanoparticles for nucleic acid amplification, which promotes the production of amplicons that accumulate on the nanoparticle surfaces, inducing significantly amplified currents during ECD that identify the presence of target genetic material. The platform, applying to the COVID-19 model, demonstrates an exceptional sensitivity of ∼1 copy/μL for 35 cycles of amplification, enabling the reduction of amplification cycles to 4 cycles (∼7 min runtime) using 1 fM complementary DNA. The nSLAM acts as an accelerator that actively promotes and participates in the nucleic acid amplification process through direct polymerization and binding of amplicons on the nanoparticle surfaces. This ultrasensitive fast-response system is a promising method for detecting emerging pathogens like the coronavirus and can be extended to detect a wider variety of biomolecules.
Keywords: DNA-functionalized nanoparticles; coronavirus; electrochemical assay; gene amplification; magnetic responsibility; molecular diagnosis; nucleic acid biosensor.
. 2023 Jan 27.
doi: 10.1021/acssensors.2c02512. Online ahead of print.
Multifunctional Nanoparticle Platform for Surface Accumulative Nucleic Acid Amplification and Rapid Electrochemical Detection: An Application to Pathogenic Coronavirus
Jeong Ook Soh[SUP] 1 2 [/SUP], Bum Chul Park[SUP] 3 4 [/SUP], Hyeon Su Park[SUP] 3 [/SUP], Myeong Soo Kim[SUP] 3 [/SUP], Hong En Fu[SUP] 3 [/SUP], Young Keun Kim[SUP] 3 4 [/SUP], Ju Hun Lee[SUP] 1 2 [/SUP]
Affiliations
- PMID: 36707063
- DOI: 10.1021/acssensors.2c02512
Abstract
Of various molecular diagnostic assays, the real-time reverse transcription polymerase chain reaction is considered the gold standard for infection diagnosis, despite critical drawbacks that limit rapid detection and accessibility. To confront these issues, several nanoparticle-based molecular detection methods have been developed to a great extent, but still possess several challenges. In this study, a novel nucleic acid amplification method termed nanoparticle-based surface localized amplification (nSLAM) is paired with electrochemical detection (ECD) to develop a nucleic acid biosensor platform that overcomes these limitations. The system uses primer-functionalized Fe[SUB]3[/SUB]O[SUB]4[/SUB]-Au core-shell nanoparticles for nucleic acid amplification, which promotes the production of amplicons that accumulate on the nanoparticle surfaces, inducing significantly amplified currents during ECD that identify the presence of target genetic material. The platform, applying to the COVID-19 model, demonstrates an exceptional sensitivity of ∼1 copy/μL for 35 cycles of amplification, enabling the reduction of amplification cycles to 4 cycles (∼7 min runtime) using 1 fM complementary DNA. The nSLAM acts as an accelerator that actively promotes and participates in the nucleic acid amplification process through direct polymerization and binding of amplicons on the nanoparticle surfaces. This ultrasensitive fast-response system is a promising method for detecting emerging pathogens like the coronavirus and can be extended to detect a wider variety of biomolecules.
Keywords: DNA-functionalized nanoparticles; coronavirus; electrochemical assay; gene amplification; magnetic responsibility; molecular diagnosis; nucleic acid biosensor.