tetano
Editor, Senior Moderator
ACS Nano
. 2024 Aug 30.
doi: 10.1021/acsnano.4c10564. Online ahead of print. Quantitatively Dissecting Triple Roles of Dynactin in Dynein-Driven Transport of Influenza Virus by Quantum Dot-Based Single-Virus Tracking
Dan-Dan Fu[SUP] 1 [/SUP], Li-Juan Zhang[SUP] 1 [/SUP], Bo Tang[SUP] 1 [/SUP], Lei Du[SUP] 1 [/SUP], Jing Li[SUP] 1 [/SUP], Jian Ao[SUP] 1 [/SUP], Zhi-Ling Zhang[SUP] 1 [/SUP], Zhi-Gang Wang[SUP] 2 [/SUP], Shu-Lin Liu[SUP] 2 [/SUP], Dai-Wen Pang[SUP] 1 2 [/SUP]
Affiliations
After entering host cells by endocytosis, influenza A virus (IAV) is transported along microfilaments and then transported by dynein along microtubules (MTs) to the perinuclear region for genome release. Understanding the mechanisms of dynein-driven transport is significant for a comprehensive understanding of IAV infection. In this work, the roles of dynactin in dynein-driven transport of IAV were quantitatively dissected in situ using quantum dot-based single-virus tracking. It was revealed that dynactin was essential for dynein to transport IAV toward the nucleus. After virus entry, virus-carrying vesicles bound to dynein and dynactin before being delivered to MTs. The attachment of dynein to the vesicles was dependent on dynactin and its subunits, p150[SUP]Glued[/SUP] and Arp1. Once viruses reached MTs, dynactin-assisted dynein initiates retrograde transport of IAV. Importantly, the retrograde transport of viruses could be initiated at both plus ends (32%) and other regions on MTs (68%). Subsequently, dynactin accompanied and assisted dynein to persistently transport the virus along MTs in the retrograde direction. This study revealed the dynactin-dependent dynein-driven transport process of IAV, enhancing our understanding of IAV infection and providing important insights into the cell's endocytic transport mechanism.
Keywords: dynactin; dynein-driven transport; influenza A virus; microtubules; single-virus tracking.
. 2024 Aug 30.
doi: 10.1021/acsnano.4c10564. Online ahead of print. Quantitatively Dissecting Triple Roles of Dynactin in Dynein-Driven Transport of Influenza Virus by Quantum Dot-Based Single-Virus Tracking
Dan-Dan Fu[SUP] 1 [/SUP], Li-Juan Zhang[SUP] 1 [/SUP], Bo Tang[SUP] 1 [/SUP], Lei Du[SUP] 1 [/SUP], Jing Li[SUP] 1 [/SUP], Jian Ao[SUP] 1 [/SUP], Zhi-Ling Zhang[SUP] 1 [/SUP], Zhi-Gang Wang[SUP] 2 [/SUP], Shu-Lin Liu[SUP] 2 [/SUP], Dai-Wen Pang[SUP] 1 2 [/SUP]
Affiliations
- PMID: 39214619
- DOI: 10.1021/acsnano.4c10564
After entering host cells by endocytosis, influenza A virus (IAV) is transported along microfilaments and then transported by dynein along microtubules (MTs) to the perinuclear region for genome release. Understanding the mechanisms of dynein-driven transport is significant for a comprehensive understanding of IAV infection. In this work, the roles of dynactin in dynein-driven transport of IAV were quantitatively dissected in situ using quantum dot-based single-virus tracking. It was revealed that dynactin was essential for dynein to transport IAV toward the nucleus. After virus entry, virus-carrying vesicles bound to dynein and dynactin before being delivered to MTs. The attachment of dynein to the vesicles was dependent on dynactin and its subunits, p150[SUP]Glued[/SUP] and Arp1. Once viruses reached MTs, dynactin-assisted dynein initiates retrograde transport of IAV. Importantly, the retrograde transport of viruses could be initiated at both plus ends (32%) and other regions on MTs (68%). Subsequently, dynactin accompanied and assisted dynein to persistently transport the virus along MTs in the retrograde direction. This study revealed the dynactin-dependent dynein-driven transport process of IAV, enhancing our understanding of IAV infection and providing important insights into the cell's endocytic transport mechanism.
Keywords: dynactin; dynein-driven transport; influenza A virus; microtubules; single-virus tracking.