tetano
Editor, Senior Moderator
ACS Cent Sci
. 2020 Nov 25;6(11):2046-2052.
doi: 10.1021/acscentsci.0c00855. Epub 2020 Sep 23.
The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
Alexander N Baker[SUP] 1 [/SUP], Sarah-Jane Richards[SUP] 1 [/SUP], Collette S Guy[SUP] 1 2 [/SUP], Thomas R Congdon[SUP] 1 [/SUP], Muhammad Hasan[SUP] 1 [/SUP], Alexander J Zwetsloot[SUP] 3 [/SUP], Angelo Gallo[SUP] 1 [/SUP], J?zef R Lewandowski[SUP] 1 [/SUP], Phillip J Stansfeld[SUP] 1 2 [/SUP], Anne Straube[SUP] 3 [/SUP], Marc Walker[SUP] 4 [/SUP], Simona Chessa[SUP] 5 [/SUP], Giulia Pergolizzi[SUP] 5 [/SUP], Simone Dedola[SUP] 5 [/SUP], Robert A Field[SUP] 5 6 [/SUP], Matthew I Gibson[SUP] 1 3 [/SUP]
Affiliations
Abstract
There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.
. 2020 Nov 25;6(11):2046-2052.
doi: 10.1021/acscentsci.0c00855. Epub 2020 Sep 23.
The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
Alexander N Baker[SUP] 1 [/SUP], Sarah-Jane Richards[SUP] 1 [/SUP], Collette S Guy[SUP] 1 2 [/SUP], Thomas R Congdon[SUP] 1 [/SUP], Muhammad Hasan[SUP] 1 [/SUP], Alexander J Zwetsloot[SUP] 3 [/SUP], Angelo Gallo[SUP] 1 [/SUP], J?zef R Lewandowski[SUP] 1 [/SUP], Phillip J Stansfeld[SUP] 1 2 [/SUP], Anne Straube[SUP] 3 [/SUP], Marc Walker[SUP] 4 [/SUP], Simona Chessa[SUP] 5 [/SUP], Giulia Pergolizzi[SUP] 5 [/SUP], Simone Dedola[SUP] 5 [/SUP], Robert A Field[SUP] 5 6 [/SUP], Matthew I Gibson[SUP] 1 3 [/SUP]
Affiliations
- PMID: 33269329
- PMCID: PMC7523238
- DOI: 10.1021/acscentsci.0c00855
Abstract
There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.