tetano
Editor, Senior Moderator
ACS Appl Bio Mater
. 2022 Jul 1.
doi: 10.1021/acsabm.2c00503. Online ahead of print.
Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen
Deepshikha Shahdeo[SUP] 1 [/SUP], Neha Chauhan[SUP] 2 3 [/SUP], Aniket Majumdar[SUP] 2 [/SUP], Arindam Ghosh[SUP] 2 4 [/SUP], Sonu Gandhi[SUP] 1 [/SUP]
Affiliations
Abstract
Coronavirus disease (COVID-19) is an infectious disease that has posed a global health challenge caused by the SARS-CoV-2 virus. Early management and diagnosis of SARS-CoV-2 are crucial for the timely treatment, traceability, and reduction of viral spread. We have developed a rapid method using a Graphene-based Field-Effect Transistor (Gr-FET) for the ultrasensitive detection of SARS-CoV-2 Spike S1 antigen (S1-Ag). The in-house developed antispike S1 antibody (S1-Ab) was covalently immobilized on the surface of a carboxy functionalized graphene channel using carbodiimide chemistry. Ultraviolet-visible spectroscopy, Fourier-Transform Infrared Spectroscopy, X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), Optical Microscopy, Raman Spectroscopy, Scanning Electron Microscopy (SEM), Enzyme-Linked Immunosorbent Assays (ELISA), and device stability studies were conducted to characterize the bioconjugation and fabrication process of Gr-FET. In addition, the electrical response of the device was evaluated by monitoring the change in resistance caused by Ag-Ab interaction in real time. For S1-Ag, our Gr-FET devices were tested in the range of 1 fM to 1 μM with a limit of detection of 10 fM in the standard buffer. The fabricated devices are highly sensitive, specific, and capable of detecting low levels of S1-Ag.
Keywords: MERS-CoV antigen; SARS-CoV-2; SARS-CoV-2 Spike S1 antigen; field-effect transistor; graphene.
. 2022 Jul 1.
doi: 10.1021/acsabm.2c00503. Online ahead of print.
Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen
Deepshikha Shahdeo[SUP] 1 [/SUP], Neha Chauhan[SUP] 2 3 [/SUP], Aniket Majumdar[SUP] 2 [/SUP], Arindam Ghosh[SUP] 2 4 [/SUP], Sonu Gandhi[SUP] 1 [/SUP]
Affiliations
- PMID: 35775242
- DOI: 10.1021/acsabm.2c00503
Abstract
Coronavirus disease (COVID-19) is an infectious disease that has posed a global health challenge caused by the SARS-CoV-2 virus. Early management and diagnosis of SARS-CoV-2 are crucial for the timely treatment, traceability, and reduction of viral spread. We have developed a rapid method using a Graphene-based Field-Effect Transistor (Gr-FET) for the ultrasensitive detection of SARS-CoV-2 Spike S1 antigen (S1-Ag). The in-house developed antispike S1 antibody (S1-Ab) was covalently immobilized on the surface of a carboxy functionalized graphene channel using carbodiimide chemistry. Ultraviolet-visible spectroscopy, Fourier-Transform Infrared Spectroscopy, X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), Optical Microscopy, Raman Spectroscopy, Scanning Electron Microscopy (SEM), Enzyme-Linked Immunosorbent Assays (ELISA), and device stability studies were conducted to characterize the bioconjugation and fabrication process of Gr-FET. In addition, the electrical response of the device was evaluated by monitoring the change in resistance caused by Ag-Ab interaction in real time. For S1-Ag, our Gr-FET devices were tested in the range of 1 fM to 1 μM with a limit of detection of 10 fM in the standard buffer. The fabricated devices are highly sensitive, specific, and capable of detecting low levels of S1-Ag.
Keywords: MERS-CoV antigen; SARS-CoV-2; SARS-CoV-2 Spike S1 antigen; field-effect transistor; graphene.