• FluTrackers.com Inc. does not provide medical advice. Information on this web site is collected from various internet resources, and the FluTrackers board of directors makes no warranty to the safety, efficacy, correctness or completeness of the information posted on this site by any author or poster. The information collated here is for instructional and/or discussion purposes only and is NOT intended to diagnose or treat any disease, illness, or other medical condition. Every individual reader or poster should seek advice from their personal physician/healthcare practitioner before considering or using any interventions that are discussed on this website. By continuing to access this website you agree to consult your personal physican before using any interventions posted on this website, and you agree to hold harmless FluTrackers.com Inc., the board of directors, the members, and all authors and posters for any effects from use of any medication, supplement, vitamin or other substance, device, intervention, etc. mentioned in posts on this website, or other internet venues referenced in posts on this website.
  • We are not asking for any donations. Do not donate to any entity who says they are raising funds for us.

A/Shanghai/60T/2009(H1N1) released 6/22

Re: A/Shanghai/60T/2009(H1N1) released 6/22

There is a chilling thought.

niman said:
Let's see. The original sequence had E627K. The first clone had E627K. That's called a confirmation. The second clone did not have E627K. Since E627K is mammalian and grows best at low temperatures (33 C), while E627 is avian and grows best at high temperatures (41 C), if the virus is being grown in eggs, there may be selection of E627 (avian version), leading to the loss of E627K during culture in eggs (but may be retained when grown on mammalian MDCK cells).

If so, then labs cloning virus in eggs may be losing E627K during culture and the level may be markedly higher than represented in the database.
Click to expand...
 
Re: A/Shanghai/60T/2009(H1N1) released 6/22

concerning the H7N7 outbreak in 2003 in the Netherlands, which caused the
culling of 30M chickens and one veterinarian had lethal infection ...

we talked about this earlier, I can't find it now.
They reported about 14 amino-acid changes in the vet.

http://www.pnas.org/content/101/5/1356.full.pdf


but in Oct.2007 they uploaded another chicken sequence, which
only has 2 nucleotide-differences:

http://www.ncbi.nlm.nih.gov/nuccore/115343242?report=genbank
VRL 01-OCT-2007
A/chicken/Netherlands/03010132/03(H7N7)
Munster,V.J., de Wit,E., Koch,G., Osterhaus,A.D.M.E. and Fouchier,R.A.M.
TITLE Determinants of enhanced replication of human H7N7 influenza A virus isolates
JOURNAL Unpublished


so there were presumably two related viruses going around with common ancestor
in late 2002,early 2003
And those mutations (except presumably E627K) did not develope in the vet

or E627K was a lab-error as in the ****** case from Shanghai ?!


for the outbreak in chickens see:
http://www.journals.uchicago.edu/doi/full/10.1086/425583
 
You must log in or register to reply here.
Back
Top