tetano
Editor, Senior Moderator
J Med Virol. 2018 Sep 20. doi: 10.1002/jmv.25322. [Epub ahead of print]
[h=1]A HRP-labeled lateral flow immunoassay for rapid simultaneous detection and differentiation of influenza A and B viruses.[/h] Zhang J[SUP]1[/SUP], Gui X[SUP]2[/SUP], Zheng Q[SUP]2[/SUP], Chen Y[SUP]2[/SUP], Ge S[SUP]2[/SUP], Zhang J[SUP]2[/SUP], Xia N[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Rapid and sensitive diagnosis of influenza is urgently needed to address the limitations of low sensitivity associated with current rapid tests available for clinics and on-site monitoring. A novel horseradish peroxidase (HRP)-labeled lateral flow immunoassay strip (HRP-LFIA) for rapid simultaneous detection and differentiation of influenza A (INF A) and influenza B (INF B) viruses was developed. This immunoassay was based on the signal amplification by the HRP-catalyzed oxidation of 3, 3,' 5, 5'-tetramethylbenzidine (TMB) forming colored insoluble product which was proportional to the analyte concentration. Compared to conventional gold-colloidal based strips, an analytical sensitivity enhancement of more than one order of magnitude for seven INF virus isolates was observed. A total of 1487 swabs obtained from persons with influenza-like illnesses (ILIs) were tested for the presence of INF A and B viruses using real-time reverse transcription polymerase chain reaction (rRT-PCR) as reference criterion. The overall sensitivities of HRP-LFIA were 77.5% (100/129) and 71.2% (116/163) for INF A and INF B, respectively. The overall specificities were 99.8% (1144/1146) and 99.8% (918/920) respectively. The nasopharyngeal sampling method yielded higher sensitivity rates of 90.2% (55/61) and 82.6% (71/86). In conclusion, this user-friendly assay could be a promising rapid detection method for rapid screening for INF A and INF B viruses. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza virus; horseradish peroxidase; lateral flow immunoassay; nucleoprotein; rapid test
PMID: 30238471 DOI: 10.1002/jmv.25322
[h=1]A HRP-labeled lateral flow immunoassay for rapid simultaneous detection and differentiation of influenza A and B viruses.[/h] Zhang J[SUP]1[/SUP], Gui X[SUP]2[/SUP], Zheng Q[SUP]2[/SUP], Chen Y[SUP]2[/SUP], Ge S[SUP]2[/SUP], Zhang J[SUP]2[/SUP], Xia N[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Rapid and sensitive diagnosis of influenza is urgently needed to address the limitations of low sensitivity associated with current rapid tests available for clinics and on-site monitoring. A novel horseradish peroxidase (HRP)-labeled lateral flow immunoassay strip (HRP-LFIA) for rapid simultaneous detection and differentiation of influenza A (INF A) and influenza B (INF B) viruses was developed. This immunoassay was based on the signal amplification by the HRP-catalyzed oxidation of 3, 3,' 5, 5'-tetramethylbenzidine (TMB) forming colored insoluble product which was proportional to the analyte concentration. Compared to conventional gold-colloidal based strips, an analytical sensitivity enhancement of more than one order of magnitude for seven INF virus isolates was observed. A total of 1487 swabs obtained from persons with influenza-like illnesses (ILIs) were tested for the presence of INF A and B viruses using real-time reverse transcription polymerase chain reaction (rRT-PCR) as reference criterion. The overall sensitivities of HRP-LFIA were 77.5% (100/129) and 71.2% (116/163) for INF A and INF B, respectively. The overall specificities were 99.8% (1144/1146) and 99.8% (918/920) respectively. The nasopharyngeal sampling method yielded higher sensitivity rates of 90.2% (55/61) and 82.6% (71/86). In conclusion, this user-friendly assay could be a promising rapid detection method for rapid screening for INF A and INF B viruses. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza virus; horseradish peroxidase; lateral flow immunoassay; nucleoprotein; rapid test
PMID: 30238471 DOI: 10.1002/jmv.25322