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Virology. 2008 Nov 11. [Epub ahead of print]
CDK/ERK-mediated phosphorylation of the human influenza A virus NS1 protein at threonine-215.
Hale BG, Knebel A, Botting CH, Galloway CS, Precious BL, Jackson D, Elliott RM, Randall RE. - Centre for Biomolecular Sciences, University of St. Andrews, North Haugh, St. Andrews, Fife KY16 9ST, UK.
Posttranslational modification of viral proteins by cellular enzymes is a feature of many virus replication strategies.
Here, we report that during infection the multifunctional human influenza A virus NS1 protein is phosphorylated at threonine-215.
Substitution of alanine for threonine at this position reduced early viral propagation, an effect apparently unrelated to NS1 antagonizing host interferon responses or activating phosphoinositide 3-kinase signaling.
In vitro, a subset of cellular proline-directed kinases, including cyclin dependent kinases (CDKs) and extracellular signal-regulated kinases (ERKs), potently phosphorylated NS1 protein at threonine-215.
Our data suggest that CDK/ERK-mediated phosphorylation of NS1 at threonine-215 is important for efficient virus replication.
PMID: 19007960 [PubMed - as supplied by publisher]
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CDK/ERK-mediated phosphorylation of the human influenza A virus NS1 protein at threonine-215.
Hale BG, Knebel A, Botting CH, Galloway CS, Precious BL, Jackson D, Elliott RM, Randall RE. - Centre for Biomolecular Sciences, University of St. Andrews, North Haugh, St. Andrews, Fife KY16 9ST, UK.
Posttranslational modification of viral proteins by cellular enzymes is a feature of many virus replication strategies.
Here, we report that during infection the multifunctional human influenza A virus NS1 protein is phosphorylated at threonine-215.
Substitution of alanine for threonine at this position reduced early viral propagation, an effect apparently unrelated to NS1 antagonizing host interferon responses or activating phosphoinositide 3-kinase signaling.
In vitro, a subset of cellular proline-directed kinases, including cyclin dependent kinases (CDKs) and extracellular signal-regulated kinases (ERKs), potently phosphorylated NS1 protein at threonine-215.
Our data suggest that CDK/ERK-mediated phosphorylation of NS1 at threonine-215 is important for efficient virus replication.
PMID: 19007960 [PubMed - as supplied by publisher]
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