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J Virol. 2011 Sep 14. [Epub ahead of print]
Mutations in the membrane proximal region of the influenza A virus M2 protein cytoplasmic tail have modest effects on virus replication.
Stewart SM, Pekosz A.
Source
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University, Bloomberg School of Public Health, 615 North Wolfe St Suite 5132, Baltimore MD 21205.
Abstract
Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. In order to systematically investigate the role of the membrane proximal residues in the M2 cytoplasmic tail on virus replication, we utilized scanning and directed alanine mutagenesis in combination with trans-complementation assays and recombinant viruses. The membrane proximal residues 46-69 tolerated numerous mutations with little, if any, affect on virus replication suggesting that protein structure, rather than individual amino acid identity in this region, may be critical for M2 protein function.
PMID:
21917980
[PubMed - as supplied by publisher]
Mutations in the membrane proximal region of the influenza A virus M2 protein cytoplasmic tail have modest effects on virus replication.
Stewart SM, Pekosz A.
Source
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University, Bloomberg School of Public Health, 615 North Wolfe St Suite 5132, Baltimore MD 21205.
Abstract
Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. In order to systematically investigate the role of the membrane proximal residues in the M2 cytoplasmic tail on virus replication, we utilized scanning and directed alanine mutagenesis in combination with trans-complementation assays and recombinant viruses. The membrane proximal residues 46-69 tolerated numerous mutations with little, if any, affect on virus replication suggesting that protein structure, rather than individual amino acid identity in this region, may be critical for M2 protein function.
PMID:
21917980
[PubMed - as supplied by publisher]
