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J Virol. 2015 Jun 3. pii: JVI.01132-15. [Epub ahead of print]
Mapping of a region of the PA-X protein of influenza A virus that is important for its shut-off activity.
Oishi K1, Yamayoshi S2, Kawaoka Y3.
Author information
Abstract
The influenza A virus PA-X comprises an N-terminal PA endonuclease domain and a C-terminal PA-X-specific domain. PA-X reduces host and viral mRNA accumulation via its endonuclease function. Here, we found that the N-terminal 15 amino acids, particularly 6 basic amino acids, in the C-terminal PA-X-specific region are important for PA-X shut-off activity. These 6 basic amino acids enabled a PA deletion mutant to suppress protein expression at a level comparable to that of wild-type PA-X.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26041295 [PubMed - as supplied by publisher]
Mapping of a region of the PA-X protein of influenza A virus that is important for its shut-off activity.
Oishi K1, Yamayoshi S2, Kawaoka Y3.
Author information
Abstract
The influenza A virus PA-X comprises an N-terminal PA endonuclease domain and a C-terminal PA-X-specific domain. PA-X reduces host and viral mRNA accumulation via its endonuclease function. Here, we found that the N-terminal 15 amino acids, particularly 6 basic amino acids, in the C-terminal PA-X-specific region are important for PA-X shut-off activity. These 6 basic amino acids enabled a PA deletion mutant to suppress protein expression at a level comparable to that of wild-type PA-X.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26041295 [PubMed - as supplied by publisher]