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Proportional Mouse Model for Aerosol Infection by Influenza

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  • Proportional Mouse Model for Aerosol Infection by Influenza

    J Appl Microbiol. 2012 Jul 19. doi: 10.1111/j.1365-2672.2012.05402.x. [Epub ahead of print]
    Proportional Mouse Model for Aerosol Infection by Influenza.
    McDonald RS, Sambol AR, Heimbuch BK, Brown TL, Hinrichs SH, Wander JD.
    Source

    Applied Research Associates, Inc., 4300 San Mateo Blvd, NE Albuquerque, NM, 87110.
    Abstract
    AIMS:

    The aim of this study was to demonstrate a prototype tool for measuring infectivity of an aerosolized human pathogen-influenza A/PR/8/34 (H1N1) virus-using a small-animal model in the Controlled Aerosol Test System (CATS).
    METHODS AND RESULTS:

    Intranasal inoculation of nonadapted H1N1 virus into C57BL, BALB/c and CD-1 mice caused infection in all three species. Respiratory exposure of CD-1 mice to the aerosolized virus at graduated doses was accomplished in a modified rodent exposure apparatus. Weight change was recorded for seven days post exposure, and viral populations in lung tissue homogenates were measured by DNA amplification (qrt-PCR), direct fluorescence and microscopic evaluation of cytopathic effect. Plots of weight change and of PCR cycle threshold vs delivered dose were linear to threshold doses of ~40 TCID(50) and ~12 TCID(50) , respectively.
    CONCLUSIONS:

    MID(50) for inspired H1N1 aerosols in CD-1 mice is between 12 and 40 TCID(50) ; proportionality to dose of weight loss and viral populations makes the CD-1 mouse a useful model for measuring infectivity by inhalation.
    SIGNIFICANCE AND IMPACT OF STUDY:

    In the CATS, this mouse-virus model provides the first quantitative method to evaluate the ability of respiratory protective technologies to attenuate the infectivity of an inspired pathogenic aerosol. ? No claim to US Government works. Journal of Applied Microbiology ? 2012 The Society for Applied Microbiology.

    ? No claim to US Government works. Journal of Applied Microbiology ? 2012 The Society for Applied Microbiology.

    PMID:
    22809111
    [PubMed - as supplied by publisher]

    http://www.ncbi.nlm.nih.gov/pubmed/22809111
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