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J Gen Virol. 2012 Feb 15. [Epub ahead of print]
The NA and M genes of the 2009 pandemic influenza H1N1 virus functionally cooperate to facilitate efficient replication and transmissibility in pigs.
Ma W, Liu Q, Bawa B, Qiao C, Qi W, Shen H, Chen Y, Ma J, Li X, Webby RJ, Garcia-Sastre A, Richt JA.
Source
Kansas State University;
Abstract
The 2009 pandemic H1N1 virus (pH1N1) contains NA and M genes from Eurasian avian-like swine influenza viruses (SIVs) and remaining 6 genes from North American triple reassortant SIVs. To characterize the role of pH1N1 NA and M genes in pathogenesis and transmission we evaluated their impact in the background of an H1N1 triple reassortant (tr1930) SIV in which we replaced the HA (H3) and NA (N2) of influenza A/swine/Texas/4199-2/98 virus with those from the classical H1N1 A/swine/Iowa/15/30 (1930) virus. The laboratory-adapted 1930 virus does not shed nor transmit in pigs, but the tr1930 was able to shed in infected pigs. We then substituted NA, M, or both NA and M genes of the tr1930 virus by those of pH1N1. The resulting virus with both NA and M from pH1N1 grew to significantly higher titers in cell cultures than the viruses with single NA or M from pH1N1. In a pig model, only the virus containing both NA and M from pH1N1 transmitted to and infected sentinels whereas the viruses with single NA or M from pH1N1 did not. Our results demonstrate that the right combination of NA and M genes is critical for replication and transmissibility of influenza viruses in pigs.
PMID:
22337640
[PubMed - as supplied by publisher]
The NA and M genes of the 2009 pandemic influenza H1N1 virus functionally cooperate to facilitate efficient replication and transmissibility in pigs.
Ma W, Liu Q, Bawa B, Qiao C, Qi W, Shen H, Chen Y, Ma J, Li X, Webby RJ, Garcia-Sastre A, Richt JA.
Source
Kansas State University;
Abstract
The 2009 pandemic H1N1 virus (pH1N1) contains NA and M genes from Eurasian avian-like swine influenza viruses (SIVs) and remaining 6 genes from North American triple reassortant SIVs. To characterize the role of pH1N1 NA and M genes in pathogenesis and transmission we evaluated their impact in the background of an H1N1 triple reassortant (tr1930) SIV in which we replaced the HA (H3) and NA (N2) of influenza A/swine/Texas/4199-2/98 virus with those from the classical H1N1 A/swine/Iowa/15/30 (1930) virus. The laboratory-adapted 1930 virus does not shed nor transmit in pigs, but the tr1930 was able to shed in infected pigs. We then substituted NA, M, or both NA and M genes of the tr1930 virus by those of pH1N1. The resulting virus with both NA and M from pH1N1 grew to significantly higher titers in cell cultures than the viruses with single NA or M from pH1N1. In a pig model, only the virus containing both NA and M from pH1N1 transmitted to and infected sentinels whereas the viruses with single NA or M from pH1N1 did not. Our results demonstrate that the right combination of NA and M genes is critical for replication and transmissibility of influenza viruses in pigs.
PMID:
22337640
[PubMed - as supplied by publisher]
