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J Gen Virol. 2012 Apr 25. [Epub ahead of print]
The use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro.
Bodewes R, Nieuwkoop NJ, Verburgh RJ, Fouchier RA, Osterhaus A, Rimmelzwaan GF.
Source
Erasmus Medical Centre, Virology.
Abstract
Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potentially pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and MDCK cells were detected by confocal microscopy and flow cytometry.
PMID:
22535774
[PubMed - as supplied by publisher]
The use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro.
Bodewes R, Nieuwkoop NJ, Verburgh RJ, Fouchier RA, Osterhaus A, Rimmelzwaan GF.
Source
Erasmus Medical Centre, Virology.
Abstract
Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potentially pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and MDCK cells were detected by confocal microscopy and flow cytometry.
PMID:
22535774
[PubMed - as supplied by publisher]
