Preparation and Detection of Glycan-Binding Activity of Influenza Virus

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Methods Mol Biol. 2020;2132:567-583. doi: 10.1007/978-1-0716-0430-4_49.
Preparation and Detection of Glycan-Binding Activity of Influenza Virus.


Nakakita SI¹, Sriwilaijaroen N², Suzuki Y³, Hirabayashi J⁴.

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Abstract

We describe a method to detect influenza virus using an evanescent-field-activated fluorescence scanner type glycan array and ELISA system. Neoglycoprotein was prepared by combination of organic chemistry and biomaterial preparation. These ligands were spotted on a glass plate or plastic well to make a glycan array and ELISA plate. We detected cultured influenza virus using glycan array and ELISA. Then, we showed that the neoglycoprotein binds to Cy3-labeled hemagglutinins (H1 and H5), a NeuAcα2,6LacNAc or NeuAcα2,3LacNAc recognized protein, as detected.



KEYWORDS:

Evanescent-field-activated fluorescence scanner; Glycan array; Hemagglutinin; Influenza virus; Neoglycoprotein


PMID:32306357DOI:10.1007/978-1-0716-0430-4_49