Announcement

Collapse
No announcement yet.

Eur Respir J . Cigarette smoke augments CSF3 expression in neutrophils to compromise alveolar-capillary barrier function during influenza infection

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Eur Respir J . Cigarette smoke augments CSF3 expression in neutrophils to compromise alveolar-capillary barrier function during influenza infection


    Eur Respir J


    . 2022 Jan 20;2102049.
    doi: 10.1183/13993003.02049-2021. Online ahead of print.
    Cigarette smoke augments CSF3 expression in neutrophils to compromise alveolar-capillary barrier function during influenza infection


    Joshua J C McGrath 1 2 3 , Gilles Vanderstocken 1 2 3 4 , Anna Dvorkin-Gheva 1 2 , Steven P Cass 1 2 , Sam Afkhami 1 2 , Matthew F Fantauzzi 1 2 , Danya Thayaparan 1 2 , Amir Reihani 5 6 , Peiyao Wang 1 2 , Ashley Beaulieu 1 2 , Pamela Shen 1 2 7 , Mathieu Morissette 8 9 , Rodrigo Jiménez-Saiz 2 10 11 12 , Spencer D Revill 5 6 , Arata Tabuchi 13 , Diana Zabini 13 , Warren L Lee 13 , Carl D Richards 1 2 , Matthew S Miller 1 14 15 , Kjetil Ask 1 2 5 , Wolfgang M Kuebler 13 16 , Jeremy A Simpson 17 , Martin R Stämpfli 18 2 5 19



    Affiliations

    Abstract

    Background: Cigarette smokers are at increased risk of acquiring influenza, developing severe disease, and requiring hospitalization/ICU admission following infection. However, immune mechanisms underlying this predisposition are incompletely understood, and therapeutic strategies for influenza are limited.
    Methods: We used a mouse model of concurrent cigarette smoke exposure and H1N1 influenza infection, colony-stimulating factor (CSF)3 supplementation/receptor (CSF3R) blockade, and single-cell RNA sequencing (scRNAseq) to investigate this relationship.
    Results: Cigarette smoke exposure exacerbated features of viral pneumonia such as edema, hypoxemia, and pulmonary neutrophilia. Smoke-exposed, infected mice demonstrated an increase in viral (v)RNA, but not replication-competent viral particles, relative to infection-only controls. Interstitial rather than airspace neutrophilia positively predicted morbidity in smoke-exposed, infected mice. Screening of pulmonary cytokines using a novel dysregulation score identified an exacerbated expression of CSF3 and interleukin (IL)-6 in the context of smoke exposure and influenza. Recombinant (r)CSF3 supplementation during influenza aggravated morbidity, hypothermia, and edema, while anti-CSF3R treatment of smoke-exposed infected mice improved alveolar-capillary barrier function. scRNAseq delineated a shift in the distribution of Csf3 + cells towards neutrophils in the context of cigarette smoke and influenza. However, although smoke-exposed lungs were enriched for infected, highly-activated neutrophils, gene signatures of these cells largely reflected an exacerbated form of typical influenza with select unique regulatory features.
    Conclusion: This work provides novel insight into the mechanisms by which cigarette smoke exacerbates influenza infection, unveiling potential therapeutic targets (e.g. excess vRNA accumulation, edematous CSF3R signaling) for use in this context, and potential limitations for clinical rCSF3 therapy during viral infectious disease.



  • #2
    bump this

    Comment

    Working...
    X