Announcement

Collapse
No announcement yet.

The interaction between NOLC1 and IAV NS1 protein promotes host cell apoptosis and reduces virus replication

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • The interaction between NOLC1 and IAV NS1 protein promotes host cell apoptosis and reduces virus replication

    Oncotarget. 2017 Oct 10;8(55):94519-94527. doi: 10.18632/oncotarget.21785. eCollection 2017 Nov 7.
    The interaction between NOLC1 and IAV NS1 protein promotes host cell apoptosis and reduces virus replication.

    Zhu C1, Zheng F1, Zhu J1, Liu M1, Liu N1, Li X1, Zhang L1, Deng Z1, Zhao Q2,3, Liu H1,2.
    Author information

    Abstract

    NS1 of the influenza virus plays an important role in the infection ability of the influenza virus. Our previous research found that NS1 protein interacts with the NOLC1 protein of host cells, however, the function of the interaction is unknown. In the present study, the role of the interaction between the two proteins in infection was further studied. Several analyses, including the use of a pull-down assay, Co-IP, western blot analysis, overexpression, RNAi, flow cytometry, etc., were used to demonstrate that the NS1 protein of H3N2 influenza virus interacts with host protein NOLC1 and reduces the quantity of NOLC1. The interaction also promotes apoptosis in A549 host cells, while the suppression of NOLC1 protein reduces the proliferation of the H3N2 virus. Based on these data, it was concluded that during the process of infection, NS1 protein interacts with NOLC1 protein, reducing the level of NOLC1, and that the interaction between the two proteins promotes apoptosis of host cells, thus reducing the proliferation of the virus. These findings provide new information on the biological function of the interaction between NS1 and NOLC1.


    KEYWORDS:

    IAV; NOLC1; NS1; protein-protein interaction; virus replication

    PMID: 29212246 PMCID: PMC5706892 DOI: 10.18632/oncotarget.21785
    Free full text
Working...
X