Key amino acid residues of neuraminidase involved in influenza A virus entry

[tetano]

Pathog Dis. 2019 Nov 8. pii: ftz063. doi: 10.1093/femspd/ftz063. [Epub ahead of print] Key amino acid residues of neuraminidase involved in influenza A virus entry.

Chen F¹, Liu T¹, Xu J¹, Huang Y¹, Liu S¹, Yang J¹.
Author information

1 Guangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China.

Abstract

Generally, influenza virus neuraminidase (NA) plays a critical role in the release stage of influenza virus. Recently, it has been found that NA may promote influenza virus to access the target cells. However, the mechanism remain unclear. Here, we reported that peramivir indeed possessed anti-influenza A virus (IAV) activity in the stage of viral entry. Importantly, we verified the critical residues of influenza NA involved in the viral entry. As a result, peramivir as an efficient NA inhibitor could suppress the initiation of IAV infection. Furthermore, mutational analysis showed NA might be associated with viral entry via amino acids residues R118, E119, D151, R152, W178, I222, E227, E276, R292 and R371. Our results demonstrated neuraminidase must contain the key amino acid residues can involve in IAV entry.
? FEMS 2019.


KEYWORDS:

Influenza A virus; neuraminidase; neuraminidase active site; peramivir; viral entry

PMID: 31702775 DOI: 10.1093/femspd/ftz063