Metabolomic Analysis of Influenza A Virus A/WSN/1933 (H1N1) Infected A549 Cells during First Cycle of Viral Replication

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Viruses. 2019 Oct 31;11(11). pii: E1007. doi: 10.3390/v11111007. Metabolomic Analysis of Influenza A Virus A/WSN/1933 (H1N1) Infected A549 Cells during First Cycle of Viral Replication.

Tian X^1,2, Zhang K³, Min J⁴, Chen C⁵, Cao Y^6,7, Ding C⁸, Liu W^9,10,11, Li J^12,13.
Author information

1 School of Life Sciences, University of Science and Technology of China, Hefei 230026, China. tienhsiaotung@foxmail.com. 2 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. tienhsiaotung@foxmail.com. 3 Philips Institute for Oral Health Research, School of Dentistry, Virginia Commonwealth University, Richmond, VA 23298, USA. kzhang@vcu.edu. 4 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. minjie00awesome@163.com. 5 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. 2008615cc@sina.com. 6 School of Life Sciences, University of Science and Technology of China, Hefei 230026, China. caoyingor@163.com. 7 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. caoyingor@163.com. 8 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China. shoveldeen@shvri.ac.cn. 9 School of Life Sciences, University of Science and Technology of China, Hefei 230026, China. liuwj@im.ac.cn. 10 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. liuwj@im.ac.cn. 11 University of Chinese Academy of Sciences, Beijing 100049, China. liuwj@im.ac.cn. 12 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. lj418@163.com. 13 University of Chinese Academy of Sciences, Beijing 100049, China. lj418@163.com.

Abstract

Influenza A virus (IAV) has developed strategies to utilize host metabolites which, after identification and isolation, can be used to discover the value of immunometabolism. During this study, to mimic the metabolic processes of influenza virus infection in human cells, we infect A549 cells with H1N1 (WSN) influenza virus and explore the metabolites with altered levels during the first cycle of influenza virus infection using ultra-high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometer (UHPLC-Q-TOF MS) technology. We annotate the metabolites using MetaboAnalyst and the Kyoto Encyclopedia of Genes and Genomes pathway analyses, which reveal that IAV regulates the abundance of the metabolic products of host cells during early infection to provide the energy and metabolites required to efficiently complete its own life cycle. These metabolites are correlated with the tricarboxylic acid (TCA) cycle and mainly are involved in purine, lipid, and glutathione metabolisms. Concurrently, the metabolites interact with signal receptors in A549 cells to participate in cellular energy metabolism signaling pathways. Metabonomic analyses have revealed that, in the first cycle, the virus not only hijacks cell metabolism for its own replication, but also affects innate immunity, indicating a need for further study of the complex relationship between IAV and host cells.


KEYWORDS:

TCA cycle; first infectious cycle; human cells; influenza virus; metabolomics analysis

PMID: 31683654 DOI: 10.3390/v11111007
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