Mol Ther Nucleic Acids. 2019 May 15;17:10-23. doi: 10.1016/j.omtn.2019.04.028. [Epub ahead of print]
Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus.

Huang SY1, Huang CH2, Chen CJ3, Chen TW4, Lin CY5, Lin YT6, Kuo SM3, Huang CG7, Lee LA8, Chen YH1, Chen MF3, Kuo RL9, Shih SR10.
Author information


The role of microRNA (miRNA) in influenza A virus (IAV) host species specificity is not well understood as yet. Here, we show that a host miRNA, miR-1290, is induced through the extracellular signal-regulated kinase (ERK) pathway upon IAV infection and is associated with increased viral titers in human cells and ferret animal models. miR-1290 was observed to target and reduce expression of the host vimentin gene. Vimentin binds with the PB2 subunit of influenza A virus ribonucleoprotein (vRNP), and knockdown of vimentin expression significantly increased vRNP nuclear retention and viral polymerase activity. Interestingly, miR-1290 was not detected in either chicken cells or mouse animal models, and the 3' UTR of the chicken vimentin gene contains no binding site for miR-1290. These findings point to a host species-specific mechanism by which IAV upregulates miR-1290 to disrupt vimentin expression and retain vRNP in the nucleus, thereby enhancing viral polymerase activity and viral replication.
Copyright 2019. Published by Elsevier Inc.


ferret; host species-specificity; influenza A virus; miR-1290; miRNA; vRNP; vimentin; viral ribonucleoprotein; virus

PMID: 31173947 PMCID: PMC6554369 DOI: 10.1016/j.omtn.2019.04.028