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Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus

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  • Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus

    Mol Ther Nucleic Acids. 2019 May 15;17:10-23. doi: 10.1016/j.omtn.2019.04.028. [Epub ahead of print]
    Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus.

    Huang SY1, Huang CH2, Chen CJ3, Chen TW4, Lin CY5, Lin YT6, Kuo SM3, Huang CG7, Lee LA8, Chen YH1, Chen MF3, Kuo RL9, Shih SR10.
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    Abstract

    The role of microRNA (miRNA) in influenza A virus (IAV) host species specificity is not well understood as yet. Here, we show that a host miRNA, miR-1290, is induced through the extracellular signal-regulated kinase (ERK) pathway upon IAV infection and is associated with increased viral titers in human cells and ferret animal models. miR-1290 was observed to target and reduce expression of the host vimentin gene. Vimentin binds with the PB2 subunit of influenza A virus ribonucleoprotein (vRNP), and knockdown of vimentin expression significantly increased vRNP nuclear retention and viral polymerase activity. Interestingly, miR-1290 was not detected in either chicken cells or mouse animal models, and the 3' UTR of the chicken vimentin gene contains no binding site for miR-1290. These findings point to a host species-specific mechanism by which IAV upregulates miR-1290 to disrupt vimentin expression and retain vRNP in the nucleus, thereby enhancing viral polymerase activity and viral replication.
    Copyright 2019. Published by Elsevier Inc.


    KEYWORDS:

    ferret; host species-specificity; influenza A virus; miR-1290; miRNA; vRNP; vimentin; viral ribonucleoprotein; virus

    PMID: 31173947 PMCID: PMC6554369 DOI: 10.1016/j.omtn.2019.04.028
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