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Cell Syst. 2018 Jun 1. pii: S2405-4712(18)30196-0. doi: 10.1016/j.cels.2018.05.008. [Epub ahead of print]
Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing.
Steuerman Y1, Cohen M2, Peshes-Yaloz N1, Valadarsky L2, Cohn O1, David E2, Frishberg A1, Mayo L1, Bacharach E1, Amit I3, Gat-Viks I4.
Author information
Abstract
The influenza virus is a major cause of morbidity and mortality worldwide. Yet, both the impact of intracellular viral replication and the variation in host response across different cell types remain uncharacterized. Here we used single-cell RNA sequencing to investigate the heterogeneity in the response of lung tissue cells to in vivo influenza infection. Analysis of viral and host transcriptomes in the same single cell enabled us to resolve the cellular heterogeneity of bystander (exposed but uninfected) as compared with infected cells. We reveal that all major immune and non-immune cell types manifest substantial fractions of infected cells, albeit at low viral transcriptome loads relative to epithelial cells. We show that all cell types respond primarily with a robust generic transcriptional response, and we demonstrate novel markers specific for influenza-infected as opposed to bystander cells. These findings open new avenues for targeted therapy aimed exclusively at infected cells.
KEYWORDS:
bystander versus infected cells; immune and non-immune cell types; influenza infection in vivo; single-cell RNA sequencing
PMID: 29886109 DOI: 10.1016/j.cels.2018.05.008
Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing.
Steuerman Y1, Cohen M2, Peshes-Yaloz N1, Valadarsky L2, Cohn O1, David E2, Frishberg A1, Mayo L1, Bacharach E1, Amit I3, Gat-Viks I4.
Author information
Abstract
The influenza virus is a major cause of morbidity and mortality worldwide. Yet, both the impact of intracellular viral replication and the variation in host response across different cell types remain uncharacterized. Here we used single-cell RNA sequencing to investigate the heterogeneity in the response of lung tissue cells to in vivo influenza infection. Analysis of viral and host transcriptomes in the same single cell enabled us to resolve the cellular heterogeneity of bystander (exposed but uninfected) as compared with infected cells. We reveal that all major immune and non-immune cell types manifest substantial fractions of infected cells, albeit at low viral transcriptome loads relative to epithelial cells. We show that all cell types respond primarily with a robust generic transcriptional response, and we demonstrate novel markers specific for influenza-infected as opposed to bystander cells. These findings open new avenues for targeted therapy aimed exclusively at infected cells.
KEYWORDS:
bystander versus infected cells; immune and non-immune cell types; influenza infection in vivo; single-cell RNA sequencing
PMID: 29886109 DOI: 10.1016/j.cels.2018.05.008