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Sheng Wu Gong Cheng Xue Bao. 2016 Apr 25;32(4):447-456. doi: 10.13345/j.cjb.150362.
[Effects of temperature and pH on the growth of H1N1 subtype of influenza A virus by surface-enhanced Raman spectroscopy].
[Article in Chinese; Abstract available in Chinese from the publisher]
Jia X1,2, Li Y1, Fan W1, Sun Q3, Zhou T4, Lin W1,2, Li J1.
Author information
Abstract
in English, Chinese
Surface enhanced Raman spectroscopy technology (SERS), using gold nanoparticles as a base, was developed for rapid and sensitive detection of virus strains. SERS can be used as a rapid and reliable method to distinguish the titers of viral replication. In the present study, we characterized H1N1 subtypes of influenza A virus strains in different conditions of pH or temperatures, while we analyzed data from SERS technology using gold nanoparticles as a base and cell cultures were employed to further confirm the data from virus strains. Origin8.0 was used to collect Raman spectra, smooth and homogenize data, and to contrast spectra. Our results indicated that the peaks of different virus strains in optimal environmental conditions (T=37 ℃/pH=7.2) reached ≥3 000. This criterion was verified by subsequent virological method. The present data indicate that the established SERS protocol can be used as a rapid and reliable method to distinguish the replication rate of virus, which can be further used in clinical samples.
KEYWORDS:
PH; SERS; influenza A virus; temperature
PMID: 28853266 DOI: 10.13345/j.cjb.150362
[Effects of temperature and pH on the growth of H1N1 subtype of influenza A virus by surface-enhanced Raman spectroscopy].
[Article in Chinese; Abstract available in Chinese from the publisher]
Jia X1,2, Li Y1, Fan W1, Sun Q3, Zhou T4, Lin W1,2, Li J1.
Author information
Abstract
in English, Chinese
Surface enhanced Raman spectroscopy technology (SERS), using gold nanoparticles as a base, was developed for rapid and sensitive detection of virus strains. SERS can be used as a rapid and reliable method to distinguish the titers of viral replication. In the present study, we characterized H1N1 subtypes of influenza A virus strains in different conditions of pH or temperatures, while we analyzed data from SERS technology using gold nanoparticles as a base and cell cultures were employed to further confirm the data from virus strains. Origin8.0 was used to collect Raman spectra, smooth and homogenize data, and to contrast spectra. Our results indicated that the peaks of different virus strains in optimal environmental conditions (T=37 ℃/pH=7.2) reached ≥3 000. This criterion was verified by subsequent virological method. The present data indicate that the established SERS protocol can be used as a rapid and reliable method to distinguish the replication rate of virus, which can be further used in clinical samples.
KEYWORDS:
PH; SERS; influenza A virus; temperature
PMID: 28853266 DOI: 10.13345/j.cjb.150362