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Vet Microbiol. 2017 Jun;205:66-70. doi: 10.1016/j.vetmic.2017.05.004. Epub 2017 May 10.
PA-X protein decreases replication and pathogenicity of swine influenza virus in cultured cells and mouse models.
Gong XQ1, Sun YF2, Ruan BY1, Liu XM1, Wang Q1, Yang HM1, Wang SY1, Zhang P1, Wang XH1, Shan TL3, Tong W3, Zhou YJ3, Li GX3, Zheng H3, Tong GZ4, Yu H5.
Author information
Abstract
Swine influenza viruses have been circulating in pigs throughout world and might be potential threats to human health. PA-X protein is a newly discovered protein produced from the PA gene by ribosomal frameshifting and the effects of PA-X on the 1918 H1N1, the pandemic 2009 H1N1, the highly pathogenic avian H5N1 and the avian H9N2 influenza viruses have been reported. However, the role of PA-X in the pathogenesis of swine influenza virus is still unknown. In this study, we rescued the H1N1 wild-type (WT) classical swine influenza virus (A/Swine/Guangdong/1/2011 (H1N1)) and H1N1 PA-X deficient virus containing mutations at the frameshift motif, and compared their replication properties and pathogenicity of swine influenza virus in vitro and in vivo. Our results show that the expression of PA-X inhibits virus replication and polymerase activity in cultured cells and decreases virulence in mouse models. Therefore, our study demonstrates that PA-X protein acts as a negative virulence regulator for classical H1N1 swine influenza virus and decreases virulence by inhibiting viral replication and polymerase activity, deepening our understanding of the pathogenesis of swine influenza virus.
Copyright ? 2017 Elsevier B.V. All rights reserved.
KEYWORDS:
Pa-x; Pathogenicity; Polymerase activity; Swine influenza virus; Virus replication
PMID: 28622865 DOI: 10.1016/j.vetmic.2017.05.004
PA-X protein decreases replication and pathogenicity of swine influenza virus in cultured cells and mouse models.
Gong XQ1, Sun YF2, Ruan BY1, Liu XM1, Wang Q1, Yang HM1, Wang SY1, Zhang P1, Wang XH1, Shan TL3, Tong W3, Zhou YJ3, Li GX3, Zheng H3, Tong GZ4, Yu H5.
Author information
Abstract
Swine influenza viruses have been circulating in pigs throughout world and might be potential threats to human health. PA-X protein is a newly discovered protein produced from the PA gene by ribosomal frameshifting and the effects of PA-X on the 1918 H1N1, the pandemic 2009 H1N1, the highly pathogenic avian H5N1 and the avian H9N2 influenza viruses have been reported. However, the role of PA-X in the pathogenesis of swine influenza virus is still unknown. In this study, we rescued the H1N1 wild-type (WT) classical swine influenza virus (A/Swine/Guangdong/1/2011 (H1N1)) and H1N1 PA-X deficient virus containing mutations at the frameshift motif, and compared their replication properties and pathogenicity of swine influenza virus in vitro and in vivo. Our results show that the expression of PA-X inhibits virus replication and polymerase activity in cultured cells and decreases virulence in mouse models. Therefore, our study demonstrates that PA-X protein acts as a negative virulence regulator for classical H1N1 swine influenza virus and decreases virulence by inhibiting viral replication and polymerase activity, deepening our understanding of the pathogenesis of swine influenza virus.
Copyright ? 2017 Elsevier B.V. All rights reserved.
KEYWORDS:
Pa-x; Pathogenicity; Polymerase activity; Swine influenza virus; Virus replication
PMID: 28622865 DOI: 10.1016/j.vetmic.2017.05.004