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Evaluation of IFITM3 rs12252 Association with Severe Pediatric Influenza Infection

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  • Evaluation of IFITM3 rs12252 Association with Severe Pediatric Influenza Infection

    J Infect Dis. 2017 May 20. doi: 10.1093/infdis/jix242. [Epub ahead of print]
    Evaluation of IFITM3 rs12252 Association with Severe Pediatric Influenza Infection.

    Randolph AG1, Yip WK2, Allen EK3, Rosenberger CM4, Agan AA5, Ash SA5, Zhang Y6, Bhangale TR7, Finkelstein D8, Cvijanovich N9, Mourani PM10, Hall M11, Su HC6, Thomas PG3; PALISI PICFLU Investigators.
    Author information

    Abstract

    Background:

    Interferon inducible transmembrane protein 3 (IFITM3) restricts endocytic fusion of influenza virus. IFITM3 rs12252_C, a putative alternate splice site, has been associated with influenza severity in adults. IFITM3 has not been evaluated in pediatric influenza.
    Methods:

    The PICFLU Study enrolled children with suspected influenza infection across 38 pediatric intensive care units November 2008 to April 2016. IFITM3 was sequenced in patients and parents were genotyped for specific variants for family-based association testing. rs12252 was genotyped in 54 African-American pediatric outpatients with influenza (FLU09), included in the population-based comparisons with 1000 genomes. Splice site analysis of rs12252_C was performed using PICFLU and FLU09 patient RNA.
    Results:

    In PICFLU, 358 children had influenza infection. We identified two rs12252_C homozygotes in 185 white non-Hispanic children. rs12252_C was not associated with influenza infection in population or family-based analyses. We did not identify the Δ21 IFITM3 isoform in RNAseq data. rs12252 genotype was not associated with IFITM3 expression levels. rs12252 was not associated with critical illness severity. No novel rare IFITM3 functional variants were identified.
    Conclusions:

    rs12252 was not associated with susceptibility to influenza-related critical illness in children or with critical illness severity. Our data also do not support it being a splice site.


    KEYWORDS:

    IFITM3; Interferon inducible transmembrane protein; genetic susceptibility; influenza; pediatric

    PMID: 28531322 DOI: 10.1093/infdis/jix242
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