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PA-X protein contributes to virulence of triple-reassortant H1N2 influenza virus by suppressing early immune responses in swine

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  • PA-X protein contributes to virulence of triple-reassortant H1N2 influenza virus by suppressing early immune responses in swine

    Virology. 2017 May 8;508:45-53. doi: 10.1016/j.virol.2017.05.002. [Epub ahead of print]
    PA-X protein contributes to virulence of triple-reassortant H1N2 influenza virus by suppressing early immune responses in swine.

    Xu G1, Zhang X2, Liu Q3, Bing G4, Hu Z2, Sun H2, Xiong X2, Jiang M2, He Q2, Wang Y2, Pu J2, Guo X2, Yang H2, Liu J2, Sun Y5.
    Author information

    Abstract

    Previous studies have identified a functional role of PA-X for influenza viruses in mice and avian species; however, its role in swine remains unknown. Toward this, we constructed PA-X deficient virus (Sw-FS) in the background of a Triple-reassortment (TR) H1N2 swine influenza virus (SIV) to assess the impact of PA-X in viral virulence in pigs. Expression of PA-X in TR H1N2 SIV enhanced viral replication and host protein synthesis shutoff, and inhibited the mRNA levels of type I IFNs and proinflammatory cytokines in porcine cells. A delay of proinflammatory responses was observed in lungs of pigs infected by wild type SIV (Sw-WT) compared to Sw-FS. Furthermore, Sw-WT virus replicated and transmitted more efficiently than Sw-FS in pigs. These results highlight the importance of PA-X in the moderation of virulence and immune responses of TR SIV in swine, which indicated that PA-X is a pro-virulence factor in TR SIV in pigs.
    Copyright ? 2017 Elsevier Inc. All rights reserved.


    KEYWORDS:

    Immune responses; PA-X protein; Swine influenza virus; Virulence

    PMID: 28494344 DOI: 10.1016/j.virol.2017.05.002
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