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Virology. 2017 Mar 31;506:99-109. doi: 10.1016/j.virol.2017.03.015. [Epub ahead of print]
Single nucleoprotein residue determines influenza A virus sensitivity to an intertypic suppression mechanism.
Narkpuk J1, Teeravechyan S1, Puthavathana P2, Jongkaewwattana A1, Jaru-Ampornpan P3.
Author information
Abstract
Several mechanisms underlying intertypic interference between co-infecting influenza types A and B viruses (IAV and IBV) have been proposed. We have recently described one in which IBV's nucleoprotein (BNP) sequestered IAV's nucleoprotein (ANP) and suppressed IAV polymerase and growth. However, its anti-IAV capacity and limitations have not been fully explored. Here, we showed that BNP's inhibitory effect was more potent toward a wide array of avian IAVs, whereas human IAVs revealed moderate resistance. BNP sensitivity was largely determined by ANP's residue 343 at the NP oligomerization interface. An avian IAV polymerase carrying an NP-V343L mutation switched from being highly BNP-sensitive to moderately BNP-resistant, and vice versa for a human IAV polymerase carrying a reverse mutation. To highlight its capacity, we demonstrated that the polymerases of highly-pathogenic H5N1 and the pandemic 2009 (H1N1) strains are strongly inhibited by BNP. Our work provides insights into lineage-specific sensitivity to BNP-mediated intertypic interference.
Copyright ? 2017 Elsevier Inc. All rights reserved.
KEYWORDS:
Avian influenza virus; Influenza virus; Intertypic interference; Nucleoprotein
PMID: 28371631 DOI: 10.1016/j.virol.2017.03.015
Single nucleoprotein residue determines influenza A virus sensitivity to an intertypic suppression mechanism.
Narkpuk J1, Teeravechyan S1, Puthavathana P2, Jongkaewwattana A1, Jaru-Ampornpan P3.
Author information
Abstract
Several mechanisms underlying intertypic interference between co-infecting influenza types A and B viruses (IAV and IBV) have been proposed. We have recently described one in which IBV's nucleoprotein (BNP) sequestered IAV's nucleoprotein (ANP) and suppressed IAV polymerase and growth. However, its anti-IAV capacity and limitations have not been fully explored. Here, we showed that BNP's inhibitory effect was more potent toward a wide array of avian IAVs, whereas human IAVs revealed moderate resistance. BNP sensitivity was largely determined by ANP's residue 343 at the NP oligomerization interface. An avian IAV polymerase carrying an NP-V343L mutation switched from being highly BNP-sensitive to moderately BNP-resistant, and vice versa for a human IAV polymerase carrying a reverse mutation. To highlight its capacity, we demonstrated that the polymerases of highly-pathogenic H5N1 and the pandemic 2009 (H1N1) strains are strongly inhibited by BNP. Our work provides insights into lineage-specific sensitivity to BNP-mediated intertypic interference.
Copyright ? 2017 Elsevier Inc. All rights reserved.
KEYWORDS:
Avian influenza virus; Influenza virus; Intertypic interference; Nucleoprotein
PMID: 28371631 DOI: 10.1016/j.virol.2017.03.015