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Cell Microbiol. 2016 Oct 3. doi: 10.1111/cmi.12679. [Epub ahead of print]
Influenza A Nucleoprotein Targets Subnuclear Structures.
H?fer CT1, Jolmes F1, Haralampiev I1, Veit M2, Herrmann A3.
Author information
Abstract
The Influenza A virus nucleoprotein (NP) is the major protein component of the genomic viral ribonucleoprotein (vRNP) complexes, which are the replication- and transcription-competent units of Influenza viruses. Early during infection, NP mediates import of vRNPs into the host cell nucleus where viral replication and transcription take place; also newly synthesized NP molecules are targeted into the nucleus, enabling coreplicational assembly of progeny vRNPs. NP reportedly acts as regulatory factor during infection, and it is known to be involved in numerous interactions with host cell proteins. Yet, the NP-host cell interplay is still poorly understood. Here, we report that NP significantly interacts with the nuclear compartment and displays distinct affinities for different subnuclear structures. NP subnuclear behavior was studied by expression of fluorescent NP fusion proteins - including obligate monomeric NP - and site-specific fluorescence photoactivation measurements. We found that NP constructs accumulate in subnuclear domains frequently found adjacent to or overlapping with PML bodies and Cajal bodies (CBs). Targeting of NP to CBs could further be demonstrated in the context of virus infection. We hypothesize that by targeting functional nuclear organization, NP might either link viral replication to specific cellular machinery or interfere with host cell processes.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Influenza A virus nucleoprotein; nuclear bodies
PMID: 27696627 DOI: 10.1111/cmi.12679
[PubMed - as supplied by publisher]
Influenza A Nucleoprotein Targets Subnuclear Structures.
H?fer CT1, Jolmes F1, Haralampiev I1, Veit M2, Herrmann A3.
Author information
Abstract
The Influenza A virus nucleoprotein (NP) is the major protein component of the genomic viral ribonucleoprotein (vRNP) complexes, which are the replication- and transcription-competent units of Influenza viruses. Early during infection, NP mediates import of vRNPs into the host cell nucleus where viral replication and transcription take place; also newly synthesized NP molecules are targeted into the nucleus, enabling coreplicational assembly of progeny vRNPs. NP reportedly acts as regulatory factor during infection, and it is known to be involved in numerous interactions with host cell proteins. Yet, the NP-host cell interplay is still poorly understood. Here, we report that NP significantly interacts with the nuclear compartment and displays distinct affinities for different subnuclear structures. NP subnuclear behavior was studied by expression of fluorescent NP fusion proteins - including obligate monomeric NP - and site-specific fluorescence photoactivation measurements. We found that NP constructs accumulate in subnuclear domains frequently found adjacent to or overlapping with PML bodies and Cajal bodies (CBs). Targeting of NP to CBs could further be demonstrated in the context of virus infection. We hypothesize that by targeting functional nuclear organization, NP might either link viral replication to specific cellular machinery or interfere with host cell processes.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Influenza A virus nucleoprotein; nuclear bodies
PMID: 27696627 DOI: 10.1111/cmi.12679
[PubMed - as supplied by publisher]