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Sci Rep
. 2024 Sep 27;14(1):22044.
doi: 10.1038/s41598-024-69635-6. RNA-Seq analysis of human heart tissue reveals SARS-CoV-2 infection and inappropriate activation of the TNF-NF-κB pathway in cardiomyocytes
Kirtan Dave 1 2 , Mukul Jain 3 4 , Meenakshi Sharma 5 , Anil Kumar Delta 5 , Chittaranjan Kole 6 , Prashant Kaushik 7
Affiliations
The negative impact of SARS-CoV-2 virus infection on cardiovascular disease (CVD) patients is well established. This research article explores the cellular pathways involved in underlying heart diseases after infection. The systemic inflammatory response to SARS-CoV-2 infection likely exacerbates this increased cardiovascular risk; however, whether the virus directly infects cardiomyocytes remains unknown due to limited multi-omics data. While public transcriptome data exists for COVID-19 infection in different cell types (including cardiomyocytes), infection times vary between studies. We used available RNA-seq data from human heart tissue to delineate SARS-CoV-2 infection and heart failure aetiology specific gene expression signatures. A total of fifty-four samples from four studies were analysed. Our aim was to investigate specific transcriptome changes occurring in cardiac tissue with SARS-CoV-2 infection compared to non-infected controls. Our data establish that SARS-CoV-2 infects cardiomyocytes by the TNF-NF-κB pathway, potentially triggering acute cardiovascular complications and increasing the long-term cardiovascular risk in COVID-19 patients.
Keywords: Bioinformatics; COVID-19; Cardiomyocytes; Gene; Heart failure; RNA-seq; TNF-NF-κB.
. 2024 Sep 27;14(1):22044.
doi: 10.1038/s41598-024-69635-6. RNA-Seq analysis of human heart tissue reveals SARS-CoV-2 infection and inappropriate activation of the TNF-NF-κB pathway in cardiomyocytes
Kirtan Dave 1 2 , Mukul Jain 3 4 , Meenakshi Sharma 5 , Anil Kumar Delta 5 , Chittaranjan Kole 6 , Prashant Kaushik 7
Affiliations
- PMID: 39333655
- PMCID: PMC11437285
- DOI: 10.1038/s41598-024-69635-6
The negative impact of SARS-CoV-2 virus infection on cardiovascular disease (CVD) patients is well established. This research article explores the cellular pathways involved in underlying heart diseases after infection. The systemic inflammatory response to SARS-CoV-2 infection likely exacerbates this increased cardiovascular risk; however, whether the virus directly infects cardiomyocytes remains unknown due to limited multi-omics data. While public transcriptome data exists for COVID-19 infection in different cell types (including cardiomyocytes), infection times vary between studies. We used available RNA-seq data from human heart tissue to delineate SARS-CoV-2 infection and heart failure aetiology specific gene expression signatures. A total of fifty-four samples from four studies were analysed. Our aim was to investigate specific transcriptome changes occurring in cardiac tissue with SARS-CoV-2 infection compared to non-infected controls. Our data establish that SARS-CoV-2 infects cardiomyocytes by the TNF-NF-κB pathway, potentially triggering acute cardiovascular complications and increasing the long-term cardiovascular risk in COVID-19 patients.
Keywords: Bioinformatics; COVID-19; Cardiomyocytes; Gene; Heart failure; RNA-seq; TNF-NF-κB.