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Nat Commun . A SARS-CoV-2 antibody curbs viral nucleocapsid protein-induced complement hyperactivation

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  • Nat Commun . A SARS-CoV-2 antibody curbs viral nucleocapsid protein-induced complement hyperactivation


    Nat Commun


    . 2021 May 11;12(1):2697.
    doi: 10.1038/s41467-021-23036-9.
    A SARS-CoV-2 antibody curbs viral nucleocapsid protein-induced complement hyperactivation


    Sisi Kang # 1 , Mei Yang # 1 , Suhua He # 1 , Yueming Wang # 2 3 , Xiaoxue Chen 1 , Yao-Qing Chen 4 , Zhongsi Hong 5 , Jing Liu 6 , Guanmin Jiang 7 , Qiuyue Chen 1 , Ziliang Zhou 1 , Zhechong Zhou 1 , Zhaoxia Huang 1 , Xi Huang 8 , Huanhuan He 1 , Weihong Zheng 2 3 , Hua-Xin Liao 9 10 , Fei Xiao 11 12 , Hong Shan 13 14 , Shoudeng Chen 15



    Affiliations

    Abstract

    Although human antibodies elicited by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein are profoundly boosted upon infection, little is known about the function of N-reactive antibodies. Herein, we isolate and profile a panel of 32 N protein-specific monoclonal antibodies (mAbs) from a quick recovery coronavirus disease-19 (COVID-19) convalescent patient who has dominant antibody responses to the SARS-CoV-2 N protein rather than to the SARS-CoV-2 spike (S) protein. The complex structure of the N protein RNA binding domain with the highest binding affinity mAb (nCoV396) reveals changes in the epitopes and antigen's allosteric regulation. Functionally, a virus-free complement hyperactivation analysis demonstrates that nCoV396 specifically compromises the N protein-induced complement hyperactivation, which is a risk factor for the morbidity and mortality of COVID-19 patients, thus laying the foundation for the identification of functional anti-N protein mAbs.


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