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Proc Natl Acad Sci U S A. 2019 Aug 9. pii: 201822046. doi: 10.1073/pnas.1822046116. [Epub ahead of print]
Long noncoding RNAs are involved in multiple immunological pathways in response to vaccination.
de Lima DS1, Cardozo LE1, Maracaja-Coutinho V2, Suhrbier A3, Mane K4, Jeffries D4, Silveira ELV1, Amaral PP5, Rappuoli R6,7, de Silva TI4,8,9, Nakaya HI10,11.
Author information
1 Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of S?o Paulo, 05508-000 S?o Paulo, Brazil. 2 Advanced Center for Chronic Diseases (ACCDiS), Facultad de Ciencias Qu?micas y Farmac?uticas, Universidad de Chile, 8380492 Santiago, Chile. 3 Inflammation Biology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4029, Australia. 4 Vaccines and Immunity Theme, Medical Research Council Unit, The Gambia at LSHTM, Banjul, The Gambia. 5 The Gurdon Institute, University of Cambridge, CB2 1QN Cambridge, United Kingdom. 6 GlaxoSmithKline, 53100 Siena, Italy; rino.r.rappuoli@gsk.com hnakaya@usp.br. 7 Department of Infectious Diseases, Imperial College London, W12 ONN London, United Kingdom. 8 Centre of International Child Health, Section of Paediatrics, Department of Medicine, Imperial College London, W2 1PG London, United Kingdom. 9 Department of Infection, Immunity & Cardiovascular Disease, University of Sheffield, S10 2RX Sheffield, United Kingdom. 10 Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of S?o Paulo, 05508-000 S?o Paulo, Brazil; rino.r.rappuoli@gsk.com hnakaya@usp.br. 11 Scientific Platform Pasteur, University of S?o Paulo, 05508-210 S?o Paulo, Brazil.
Abstract
Understanding the mechanisms of vaccine-elicited protection contributes to the development of new vaccines. The emerging field of systems vaccinology provides detailed information on host responses to vaccination and has been successfully applied to study the molecular mechanisms of several vaccines. Long noncoding RNAs (lncRNAs) are crucially involved in multiple biological processes, but their role in vaccine-induced immunity has not been explored. We performed an analysis of over 2,000 blood transcriptome samples from 17 vaccine cohorts to identify lncRNAs potentially involved with antibody responses to influenza and yellow fever vaccines. We have created an online database where all results from this analysis can be accessed easily. We found that lncRNAs participate in distinct immunological pathways related to vaccine-elicited responses. Among them, we showed that the expression of lncRNA FAM30A was high in B cells and correlates with the expression of immunoglobulin genes located in its genomic vicinity. We also identified altered expression of these lncRNAs in RNA-sequencing (RNA-seq) data from a cohort of children following immunization with intranasal live attenuated influenza vaccine, suggesting a common role across several diverse vaccines. Taken together, these findings provide evidence that lncRNAs have a significant impact on immune responses induced by vaccination.
Copyright ? 2019 the Author(s). Published by PNAS.
KEYWORDS:
long noncoding RNAs; systems biology; transcriptome; vaccination
PMID: 31399544 DOI: 10.1073/pnas.1822046116
Free full text
Long noncoding RNAs are involved in multiple immunological pathways in response to vaccination.
de Lima DS1, Cardozo LE1, Maracaja-Coutinho V2, Suhrbier A3, Mane K4, Jeffries D4, Silveira ELV1, Amaral PP5, Rappuoli R6,7, de Silva TI4,8,9, Nakaya HI10,11.
Author information
1 Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of S?o Paulo, 05508-000 S?o Paulo, Brazil. 2 Advanced Center for Chronic Diseases (ACCDiS), Facultad de Ciencias Qu?micas y Farmac?uticas, Universidad de Chile, 8380492 Santiago, Chile. 3 Inflammation Biology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4029, Australia. 4 Vaccines and Immunity Theme, Medical Research Council Unit, The Gambia at LSHTM, Banjul, The Gambia. 5 The Gurdon Institute, University of Cambridge, CB2 1QN Cambridge, United Kingdom. 6 GlaxoSmithKline, 53100 Siena, Italy; rino.r.rappuoli@gsk.com hnakaya@usp.br. 7 Department of Infectious Diseases, Imperial College London, W12 ONN London, United Kingdom. 8 Centre of International Child Health, Section of Paediatrics, Department of Medicine, Imperial College London, W2 1PG London, United Kingdom. 9 Department of Infection, Immunity & Cardiovascular Disease, University of Sheffield, S10 2RX Sheffield, United Kingdom. 10 Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of S?o Paulo, 05508-000 S?o Paulo, Brazil; rino.r.rappuoli@gsk.com hnakaya@usp.br. 11 Scientific Platform Pasteur, University of S?o Paulo, 05508-210 S?o Paulo, Brazil.
Abstract
Understanding the mechanisms of vaccine-elicited protection contributes to the development of new vaccines. The emerging field of systems vaccinology provides detailed information on host responses to vaccination and has been successfully applied to study the molecular mechanisms of several vaccines. Long noncoding RNAs (lncRNAs) are crucially involved in multiple biological processes, but their role in vaccine-induced immunity has not been explored. We performed an analysis of over 2,000 blood transcriptome samples from 17 vaccine cohorts to identify lncRNAs potentially involved with antibody responses to influenza and yellow fever vaccines. We have created an online database where all results from this analysis can be accessed easily. We found that lncRNAs participate in distinct immunological pathways related to vaccine-elicited responses. Among them, we showed that the expression of lncRNA FAM30A was high in B cells and correlates with the expression of immunoglobulin genes located in its genomic vicinity. We also identified altered expression of these lncRNAs in RNA-sequencing (RNA-seq) data from a cohort of children following immunization with intranasal live attenuated influenza vaccine, suggesting a common role across several diverse vaccines. Taken together, these findings provide evidence that lncRNAs have a significant impact on immune responses induced by vaccination.
Copyright ? 2019 the Author(s). Published by PNAS.
KEYWORDS:
long noncoding RNAs; systems biology; transcriptome; vaccination
PMID: 31399544 DOI: 10.1073/pnas.1822046116
Free full text