Sci Immunol. 2019 Apr 19;4(34). pii: eaau2710. doi: 10.1126/sciimmunol.aau2710.
Prolonged evolution of the memory B cell response induced by a replicating adenovirus-influenza H5 vaccine.
Matsuda K1, Huang J1, Zhou T2, Sheng Z3, Kang BH1, Ishida E1, Griesman T1, Stuccio S1, Bolkhovitinov L1, Wohlbold TJ4, Chromikova V4, Cagigi A2, Leung K2, Andrews S2, Cheung CSF2, Pullano AA1, Plyler J2, Soto C2, Zhang B2, Yang Y2, Joyce MG2, Tsybovsky Y5, Wheatley A2, Narpala SR2, Guo Y3, Darko S2, Bailer RT2, Poole A1, Liang CJ6, Smith J7, Alexander J7, Gurwith M7, Migueles SA1, Koup RA2, Golding H8, Khurana S8, McDermott AB2, Shapiro L3, Krammer F4, Kwong PD2, Connors M9.
Author information
Abstract
Induction of an antibody response capable of recognizing highly diverse strains is a major obstacle to the development of vaccines for viruses such as HIV and influenza. Here, we report the dynamics of B cell expansion and evolution at the single-cell level after vaccination with a replication-competent adenovirus type 4 recombinant virus expressing influenza H5 hemagglutinin. Fluorescent H1 or H5 probes were used to quantitate and isolate peripheral blood B cells and their antigen receptors. We observed increases in H5-specific antibody somatic hypermutation and potency for several months beyond the period of active viral replication that was not detectable at the serum level. Individual broad and potent antibodies could be isolated, including one stem-specific antibody that is part of a new multidonor class. These results demonstrate prolonged evolution of the B cell response for months after vaccination and should be considered in efforts to evaluate or boost vaccine-induced immunity.
Copyright ? 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMID: 31004012 DOI: 10.1126/sciimmunol.aau2710
Prolonged evolution of the memory B cell response induced by a replicating adenovirus-influenza H5 vaccine.
Matsuda K1, Huang J1, Zhou T2, Sheng Z3, Kang BH1, Ishida E1, Griesman T1, Stuccio S1, Bolkhovitinov L1, Wohlbold TJ4, Chromikova V4, Cagigi A2, Leung K2, Andrews S2, Cheung CSF2, Pullano AA1, Plyler J2, Soto C2, Zhang B2, Yang Y2, Joyce MG2, Tsybovsky Y5, Wheatley A2, Narpala SR2, Guo Y3, Darko S2, Bailer RT2, Poole A1, Liang CJ6, Smith J7, Alexander J7, Gurwith M7, Migueles SA1, Koup RA2, Golding H8, Khurana S8, McDermott AB2, Shapiro L3, Krammer F4, Kwong PD2, Connors M9.
Author information
Abstract
Induction of an antibody response capable of recognizing highly diverse strains is a major obstacle to the development of vaccines for viruses such as HIV and influenza. Here, we report the dynamics of B cell expansion and evolution at the single-cell level after vaccination with a replication-competent adenovirus type 4 recombinant virus expressing influenza H5 hemagglutinin. Fluorescent H1 or H5 probes were used to quantitate and isolate peripheral blood B cells and their antigen receptors. We observed increases in H5-specific antibody somatic hypermutation and potency for several months beyond the period of active viral replication that was not detectable at the serum level. Individual broad and potent antibodies could be isolated, including one stem-specific antibody that is part of a new multidonor class. These results demonstrate prolonged evolution of the B cell response for months after vaccination and should be considered in efforts to evaluate or boost vaccine-induced immunity.
Copyright ? 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMID: 31004012 DOI: 10.1126/sciimmunol.aau2710