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Vaccine. 2019 Mar 8. pii: S0264-410X(19)30264-6. doi: 10.1016/j.vaccine.2019.02.057. [Epub ahead of print]
Inactivated whole virus particle vaccine with potent immunogenicity and limited IL-6 induction is ideal for influenza.
Sekiya T1, Mifsud EJ1, Ohno M2, Nomura N2, Sasada M2, Fujikura D2, Daito T2, Shingai M3, Ohara Y4, Nishimura T4, Endo M4, Mitsumata R5, Ikeda T5, Hatanaka H6, Kitayama H6, Motokawa K7, Sobue T8, Suzuki S9, Itoh Y9, Brown LE10, Ogasawara K11, Kino Y12, Kida H13.
Author information
Abstract
In contrast to current ether- or detergent-disrupted "split" vaccines (SVs) for influenza, inactivated whole influenza virus particle vaccines (WPVs) retain the original virus structure and components and as such may confer similar immunity to natural infection. In a collaboration between academia and industry, the potential of WPV as a new seasonal influenza vaccine was investigated. Each of the four seasonal influenza vaccine manufacturers in Japan prepared WPVs and SVs from the same batches of purified influenza virus. Both mice and monkeys vaccinated with the WPVs exhibited superior immune responses to those vaccinated with the corresponding SVs. Vaccination with A/California/07/2009 (H1N1) WPV enabled mice to survive a lethal challenge dose of homologous virus whereas those vaccinated with SV succumbed to infection within 6 days. Furthermore, mice vaccinated with WPV induced substantial numbers of multifunctional CD8+ T cells, important for control of antigenically drifted influenza virus strains. In addition, cytokines and chemokines were detected at early time points in the sera of mice vaccinated with WPV but not in those animals vaccinated with SV. These results indicate that WPVs induce enhanced innate and adaptive immune responses compared to equivalent doses of SVs. Notably, WPV at one fifth of the dose of SV was able to induce potent immunity with limited production of IL-6, one of the pyrogenic cytokines. We thus propose that WPVs with balanced immunogenicity and safety may set a new global standard for seasonal influenza vaccines.
Copyright ? 2019. Published by Elsevier Ltd.
KEYWORDS:
Inactivated whole influenza virus particle vaccine; Seasonal and pandemic influenza
PMID: 30857932 DOI: 10.1016/j.vaccine.2019.02.057
Inactivated whole virus particle vaccine with potent immunogenicity and limited IL-6 induction is ideal for influenza.
Sekiya T1, Mifsud EJ1, Ohno M2, Nomura N2, Sasada M2, Fujikura D2, Daito T2, Shingai M3, Ohara Y4, Nishimura T4, Endo M4, Mitsumata R5, Ikeda T5, Hatanaka H6, Kitayama H6, Motokawa K7, Sobue T8, Suzuki S9, Itoh Y9, Brown LE10, Ogasawara K11, Kino Y12, Kida H13.
Author information
Abstract
In contrast to current ether- or detergent-disrupted "split" vaccines (SVs) for influenza, inactivated whole influenza virus particle vaccines (WPVs) retain the original virus structure and components and as such may confer similar immunity to natural infection. In a collaboration between academia and industry, the potential of WPV as a new seasonal influenza vaccine was investigated. Each of the four seasonal influenza vaccine manufacturers in Japan prepared WPVs and SVs from the same batches of purified influenza virus. Both mice and monkeys vaccinated with the WPVs exhibited superior immune responses to those vaccinated with the corresponding SVs. Vaccination with A/California/07/2009 (H1N1) WPV enabled mice to survive a lethal challenge dose of homologous virus whereas those vaccinated with SV succumbed to infection within 6 days. Furthermore, mice vaccinated with WPV induced substantial numbers of multifunctional CD8+ T cells, important for control of antigenically drifted influenza virus strains. In addition, cytokines and chemokines were detected at early time points in the sera of mice vaccinated with WPV but not in those animals vaccinated with SV. These results indicate that WPVs induce enhanced innate and adaptive immune responses compared to equivalent doses of SVs. Notably, WPV at one fifth of the dose of SV was able to induce potent immunity with limited production of IL-6, one of the pyrogenic cytokines. We thus propose that WPVs with balanced immunogenicity and safety may set a new global standard for seasonal influenza vaccines.
Copyright ? 2019. Published by Elsevier Ltd.
KEYWORDS:
Inactivated whole influenza virus particle vaccine; Seasonal and pandemic influenza
PMID: 30857932 DOI: 10.1016/j.vaccine.2019.02.057