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Int J Nanomedicine. 2018 Oct 24;13:6699-6715. doi: 10.2147/IJN.S178809. eCollection 2018.
Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs.
Dhakal S1,2, Cheng X3, Salcido J3, Renu S1,2, Bondra K1,2, Lakshmanappa YS1,2, Misch C1,2, Ghimire S1,2, Feliciano-Ruiz N1,2, Hogshead B1,2, Krakowka S4, Carson K3, McDonough J3, Lee CW1,2, Renukaradhya GJ1,2.
Author information
Abstract
Background:
Influenza (flu) is a constant threat to humans and animals, and vaccination is one of the most effective ways to mitigate the disease. Due to incomplete protection induced by current flu vaccines, development of novel flu vaccine candidates is warranted to achieve greater efficacy against constantly evolving flu viruses.
Methods:
In the present study, we used liposome nanoparticle (<200 nm diameter)-based subunit flu vaccine containing ten encapsulated highly conserved B and T cell epitope peptides to induce protective immune response against a zoonotic swine influenza A virus (SwIAV) H1N1 challenge infection in a pig model. Furthermore, we used monosodium urate (MSU) crystals as an adjuvant and co-administered the vaccine formulation as an intranasal mist to flu-free nursery pigs, twice at 3-week intervals.
Results:
Liposome peptides flu vaccine delivered with MSU adjuvant improved the hemagglutination inhibition antibody titer and mucosal IgA response against the SwIAV challenge and also against two other highly genetically variant IAVs. Liposomal vaccines also enhanced the frequency of peptides and virus-specific T-helper/memory cells and IFN-γ response. The improved specific cellular and mucosal humoral immune responses in adjuvanted liposomal peptides flu vaccine partially protected pigs from flu-induced fever and pneumonic lesions, and reduced the nasal virus shedding and viral load in the lungs.
Conclusion:
Overall, our study shows great promise for using liposome and MSU adjuvant- based subunit flu vaccine through the intranasal route, and provides scope for future, pre-clinical investigations in a pig model for developing potent human intranasal subunit flu vaccines.
KEYWORDS:
influenza A virus peptides; intranasal vaccination; liposome nanoparticles; monosodium urate crystal adjuvant; pigs; swine influenza virus
PMID: 30425484 PMCID: PMC6205527 DOI: 10.2147/IJN.S178809
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Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs.
Dhakal S1,2, Cheng X3, Salcido J3, Renu S1,2, Bondra K1,2, Lakshmanappa YS1,2, Misch C1,2, Ghimire S1,2, Feliciano-Ruiz N1,2, Hogshead B1,2, Krakowka S4, Carson K3, McDonough J3, Lee CW1,2, Renukaradhya GJ1,2.
Author information
Abstract
Background:
Influenza (flu) is a constant threat to humans and animals, and vaccination is one of the most effective ways to mitigate the disease. Due to incomplete protection induced by current flu vaccines, development of novel flu vaccine candidates is warranted to achieve greater efficacy against constantly evolving flu viruses.
Methods:
In the present study, we used liposome nanoparticle (<200 nm diameter)-based subunit flu vaccine containing ten encapsulated highly conserved B and T cell epitope peptides to induce protective immune response against a zoonotic swine influenza A virus (SwIAV) H1N1 challenge infection in a pig model. Furthermore, we used monosodium urate (MSU) crystals as an adjuvant and co-administered the vaccine formulation as an intranasal mist to flu-free nursery pigs, twice at 3-week intervals.
Results:
Liposome peptides flu vaccine delivered with MSU adjuvant improved the hemagglutination inhibition antibody titer and mucosal IgA response against the SwIAV challenge and also against two other highly genetically variant IAVs. Liposomal vaccines also enhanced the frequency of peptides and virus-specific T-helper/memory cells and IFN-γ response. The improved specific cellular and mucosal humoral immune responses in adjuvanted liposomal peptides flu vaccine partially protected pigs from flu-induced fever and pneumonic lesions, and reduced the nasal virus shedding and viral load in the lungs.
Conclusion:
Overall, our study shows great promise for using liposome and MSU adjuvant- based subunit flu vaccine through the intranasal route, and provides scope for future, pre-clinical investigations in a pig model for developing potent human intranasal subunit flu vaccines.
KEYWORDS:
influenza A virus peptides; intranasal vaccination; liposome nanoparticles; monosodium urate crystal adjuvant; pigs; swine influenza virus
PMID: 30425484 PMCID: PMC6205527 DOI: 10.2147/IJN.S178809
Free full text