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Identification of immunodominant CD8 epitope in the stalk domain of influenza B viral hemagglutinin

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  • Identification of immunodominant CD8 epitope in the stalk domain of influenza B viral hemagglutinin

    Biochem Biophys Res Commun. 2018 May 21. pii: S0006-291X(18)31217-8. doi: 10.1016/j.bbrc.2018.05.148. [Epub ahead of print]
    Identification of immunodominant CD8 epitope in the stalk domain of influenza B viral hemagglutinin.

    Muralidharan A1, Gravel C2, Duran A1, Larocque L2, Li C3, Zetner A4, Van Domselaar G4, Wang L5, Li X6.
    Author information

    Abstract

    Human infections by type B influenza virus constitute about 25% of all influenza cases. The viral hemagglutinin is comprised of two subunits, HA1 and HA2. While HA1 is constantly evolving in an unpredictable fashion, the HA2 subunit is highly conserved, making it a potential candidate for a universal vaccine. However, immunodominant epitopes in the HA2 subunit remain largely unknown. To delineate MHC Class I epitopes, we first identified 9-mer H-2Kd-restricted CD8 T cell epitopes in the HA2 domain by in silico analyses, followed by evaluating the immunodominance of these peptides in mice challenged with the virus. Of three peptides selected through in silico analysis, the universally conserved peptide, YYSTAASSL (B/HA2-190), possessed the highest predicted binding affinity to MHC Class I and was most effective in inducing IL-2 and TNF-α in mouse splenocytes. Importantly, the peptide demonstrated best capability of stimulating peptide-specific ex-vivo cytotoxicity against target cells. Taken together, this finding would be of value for assessment of cell-mediated immune responses elicited by vaccines based on the highly conserved HA2 stalk domain.


    KEYWORDS:

    Hemagglutinin; Influenza; MHC class I; T cell epitope; Vaccine

    PMID: 29792863 DOI: 10.1016/j.bbrc.2018.05.148
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