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Cell Immunol. 2018 Apr 11. pii: S0008-8749(18)30075-3. doi: 10.1016/j.cellimm.2018.04.005. [Epub ahead of print]
Intranasal delivery of influenza antigen by nanoparticles, but not NKT-cell adjuvant differentially induces the expression of B-cell activation factors in mice and swine.
Renu S1, Dhakal S1, Kim E2, Goodman J3, Lakshmanappa YS1, Wannemuehler MJ4, Narasimhan B3, Boyaka PN2, Renukaradhya GJ5.
Author information
Abstract
Intranasal vaccination of pigs with poly lactic-co-glycolic acid and polyanhydride nanoparticles delivered inactivated influenza virus provides cross-reactive T-cell response, but not antibody response, resulting in incomplete protection and no reduction in nasal virus shedding. Expression of BAFF and Th2 transcription factor GATA-3 were downregulated in lungs of pigs vaccinated with influenza nanovaccine, but in mice it upregulated the expression of BAFF and cytokine TGFβ in cervical lymph nodes. However, the intranasal iNKT cell adjuvant, α-Galctosylceramide upregulates the expression of BAFF in pig lungs. In conclusion, expression of BAFF is differentially regulated by intranasal nanovaccine and α-Galctosylceramide in pig respiratory tract.
KEYWORDS:
Antibodies; B-cell activation factors; Mice; Nanoparticles; Pigs; iNKT cells
PMID: 29665972 DOI: 10.1016/j.cellimm.2018.04.005
Intranasal delivery of influenza antigen by nanoparticles, but not NKT-cell adjuvant differentially induces the expression of B-cell activation factors in mice and swine.
Renu S1, Dhakal S1, Kim E2, Goodman J3, Lakshmanappa YS1, Wannemuehler MJ4, Narasimhan B3, Boyaka PN2, Renukaradhya GJ5.
Author information
Abstract
Intranasal vaccination of pigs with poly lactic-co-glycolic acid and polyanhydride nanoparticles delivered inactivated influenza virus provides cross-reactive T-cell response, but not antibody response, resulting in incomplete protection and no reduction in nasal virus shedding. Expression of BAFF and Th2 transcription factor GATA-3 were downregulated in lungs of pigs vaccinated with influenza nanovaccine, but in mice it upregulated the expression of BAFF and cytokine TGFβ in cervical lymph nodes. However, the intranasal iNKT cell adjuvant, α-Galctosylceramide upregulates the expression of BAFF in pig lungs. In conclusion, expression of BAFF is differentially regulated by intranasal nanovaccine and α-Galctosylceramide in pig respiratory tract.
KEYWORDS:
Antibodies; B-cell activation factors; Mice; Nanoparticles; Pigs; iNKT cells
PMID: 29665972 DOI: 10.1016/j.cellimm.2018.04.005