Announcement

Collapse
No announcement yet.

Intranasal live influenza vaccine priming elicits localized B cell responses in mediastinal lymph nodes

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Intranasal live influenza vaccine priming elicits localized B cell responses in mediastinal lymph nodes

    J Virol. 2018 Feb 14. pii: JVI.01970-17. doi: 10.1128/JVI.01970-17. [Epub ahead of print]
    Intranasal live influenza vaccine priming elicits localized B cell responses in mediastinal lymph nodes.

    Jegaskanda S1,2, Mason RD3, Andrews SF3, Wheatley AK3,2, Zhang R4, Reynoso G5, Ambrozak D3, Santos C1, Luke C1, Matsuoka Y1, Brenchley JM6, Hickman HD5, Talaat K7, Permar SR4, Liao HX4, Yewdell JW5, Koup RA3, Roederer M3, McDermott AB8, Subbarao K9.
    Author information

    Abstract

    Pandemic live attenuated influenza vaccines (pLAIV) prime subjects for a robust neutralizing antibody response upon subsequent administration of inactivated subunit vaccine (pISV). However, a difference was not detected in H5-specific memory B cells in the peripheral blood between pLAIV-primed and unprimed subjects prior to pISV boost. To investigate the mechanism underlying pLAIV priming, we vaccinated groups of 12 African green monkeys (AGMs) with H5N1 pISV or pLAIV alone or H5N1 pLAIV followed by pISV and examined immunity systemically and in local draining lymph nodes (LN). The AGM model recapitulated the serologic observations from clinical studies. Interestingly, H5N1 pLAIV induced robust germinal center B cell responses in the mediastinal LN (MLN). Subsequent boosting with H5N1 pISV drove increases in H5-specific B cells in the axillary LNs, spleen and circulation in H5N1 pLAIV-primed animals. Thus, H5N1 pLAIV primes localized B cell responses in the MLN that are recalled systemically following pISV boost. These data provide mechanistic insights for the generation of robust humoral responses via prime-boost vaccination.IMPORTANCE: We have previously shown that pandemic live attenuated influenza viruses (pLAIV), prime for a rapid and robust antibody response on subsequent administration of inactivated subunit vaccine (pISV). This is observed even in individuals who had undetectable Ab responses following the initial vaccination. To define the mechanistic basis of pLAIV priming, we turned to a non-human primate model and performed a detailed analysis of B cell responses in systemic and local lymphoid tissues following prime-boost vaccination with pLAIV and pISV. We show that the non-human primate model recapitulates the serologic observations from clinical studies. Further, we found that pLAIV induced robust germinal center B cell responses in the mediastinal lymph node. Subsequent boosting with pISV in pLAIV-primed animals resulted in detection of B cells in the axillary lymph nodes, spleen and peripheral blood. We demonstrate that intranasally administered pLAIV elicits a highly localized germinal center B cell response in the mediastinal lymph node, that is rapidly recalled following pISV boost into germinal center reactions at numerous distant immune sites.


    PMID: 29444938 DOI: 10.1128/JVI.01970-17
Working...
X