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Virology. 2017 Dec 7;515:21-28. doi: 10.1016/j.virol.2017.11.018. [Epub ahead of print]
A novel peptide-based vaccine candidate with protective efficacy against influenza A in a mouse model.
Herrera-Rodriguez J1, Meijerhof T1, Niesters HG1, Stjernholm G2, Hovden AO2, S?rensen B3, ?kvist M2, Sommerfelt MA2, Huckriede A4.
Author information
Abstract
Current influenza vaccines mainly induce antibody responses to the variable hemagglutinin proteins of the virus strains included in the vaccine. Instead, a broadly protective influenza vaccine should aim at inducing antibody- and/or cell-mediated immunity against conserved viral proteins. Vacc-FLU is a peptide based vaccine combining conserved B and T cell epitopes. Peptide selection was done using a proprietary peptide design platform technology focusing on responses to human leukocyte antigen (HLA)-restricted epitopes. Immunization of wild-type mice and mice transgenic for HLA-A2.1 with the peptide mix successfully induced both humoral and cell mediated immune responses. Partial protection from severe weight loss upon challenge was observed in both mouse strains but was stronger and observed at lower vaccine doses in transgenic mice. Our results show that the Vacc-FLU peptide mix is capable of inducing IFNγ-producing T cells and antibody-producing B cells which can protect from severe disease symptoms upon infection.
KEYWORDS:
Cell mediated immunity; Humoral immunity; Influenza; Peptides; Universal vaccine
PMID: 29223787 DOI: 10.1016/j.virol.2017.11.018
A novel peptide-based vaccine candidate with protective efficacy against influenza A in a mouse model.
Herrera-Rodriguez J1, Meijerhof T1, Niesters HG1, Stjernholm G2, Hovden AO2, S?rensen B3, ?kvist M2, Sommerfelt MA2, Huckriede A4.
Author information
Abstract
Current influenza vaccines mainly induce antibody responses to the variable hemagglutinin proteins of the virus strains included in the vaccine. Instead, a broadly protective influenza vaccine should aim at inducing antibody- and/or cell-mediated immunity against conserved viral proteins. Vacc-FLU is a peptide based vaccine combining conserved B and T cell epitopes. Peptide selection was done using a proprietary peptide design platform technology focusing on responses to human leukocyte antigen (HLA)-restricted epitopes. Immunization of wild-type mice and mice transgenic for HLA-A2.1 with the peptide mix successfully induced both humoral and cell mediated immune responses. Partial protection from severe weight loss upon challenge was observed in both mouse strains but was stronger and observed at lower vaccine doses in transgenic mice. Our results show that the Vacc-FLU peptide mix is capable of inducing IFNγ-producing T cells and antibody-producing B cells which can protect from severe disease symptoms upon infection.
KEYWORDS:
Cell mediated immunity; Humoral immunity; Influenza; Peptides; Universal vaccine
PMID: 29223787 DOI: 10.1016/j.virol.2017.11.018